| 利用RAPD技术鉴定大白菜主栽品种及品种间遗传多样性分析 |
| |
| 引用本文： | 宋顺华,郑晓鹰.利用RAPD技术鉴定大白菜主栽品种及品种间遗传多样性分析[J].华北农学报,2000,15(3):1-5. |
| |
| 作者姓名： | 宋顺华 郑晓鹰 |
| |
| 作者单位： | 北京市蔬菜研究中心,北京,100089 |
| |
| 摘 要： | 采用随机引物扩增多态ＤＮＡ（ＲＡＰＤ）技术分析了２１个大白菜主栽品种。用１３个引物共扩增出８７个清晰可重复的ＤＮＡ片段,其中３９条带具有多态性,多态性条带的频率为４４．８％。ＯＰＥ０１是多态性频率最高的引物,它可区分１５个大白菜品种,再与引物ＯＰＨ０３和ＯＰＨ１２配合,即可将２１个大白菜品种区别开。
|
| 关 键 词： | 大白菜 主栽品种 RAPD标记 遗传多样性 品种鉴定 |
| 修稿时间： | 1999-05-25 |
Identification of Main Chinese Cabbage Cultivars with Random Amplified Polymorphic DNA Markers |
| |
| SONG Shun-hua,ZHENG Xiao-ying.Identification of Main Chinese Cabbage Cultivars with Random Amplified Polymorphic DNA Markers[J].Acta Agriculturae Boreali-Sinica,2000,15(3):1-5. |
| |
| Authors: | SONG Shun-hua ZHENG Xiao-ying |
| |
| Abstract: | Twenty one Chinese cabbage cultivars were analyzed with Random amplified polymorphic DNA(RAPD).Using 13 arbitrary 10 mer primers,we scored a total of 87 clear and repeatable different fragments for their presence/absence.39 bands (44.8%)were polymorphic.Primer OPE 01 had the highest ratio of polymorphic,with which 15 Chinese cabbage cultivars could be distinguished.Primer OPE 01 combined with primer OPH 03 and primer OPH 12 could distinguish all of the Chinese cabbage cultivars. |
| |
| Keywords: | Chinese cabbage Main cultivars RAPD markers |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
