Decrease of Waxy (Wx) Protein in Two Common Wheat Cultivars with Low Amylose Content |
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Authors: | T. Nakamura M. Yamamori H. Hirano S. Hidaka |
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Affiliation: | Tohoku National Agricultural Experiment Station, Akahira 4, Simokuriyagawa, Morioka, Iwate, 020-01, Japan;Tropical Agriculture Research Centre, Okinawa Branch, Ishigaki, Okinawa 907, Japan;National Institute of Agrobiological Resources, Kannondai 2-1-2, Tukuba, Ibaraki, 305, Japan. |
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Abstract: | Two cultivars with a low amylose content, Kanto107 (K107) and K79, were discovered in Japanese common wheat (Triticum aestivum L.). The amount of Wx protein, identified as a single major starch granule-bound protein of about 61 kD by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), was greatly decreased in those two cultivars. Analysis of their Wx protein with a modified SDS-PAGE and two dimensional-PAGE showed that two of the three Wx proteins, produced by Wx-A1 and Wx-B1 loci, were not detected. It was thus concluded that only one locus, Wx-D1, was active in the two low amylose cultivars. These mutants were termed “partial waxy mutants” and considered to be very useful material for breeding waxy wheat. |
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Keywords: | Triticum aestivum amylose content Wx protein waxy mutant |
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