| RP-HPLC法测定健胃散中枸橼酸含量 |
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| 引用本文： | 金录胜,李树纲,王登临,史梓萱,张宏,张蕾,武生平,张旭.RP-HPLC法测定健胃散中枸橼酸含量[J].中兽医医药杂志,2012,31(2):42-44. |
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| 作者姓名： | 金录胜 李树纲 王登临 史梓萱 张宏 张蕾 武生平 张旭 |
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| 作者单位： | 甘肃省兽药饲料监察所,甘肃兰州,730030 |
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| 摘 要： | 建立RP-HPLC法测定健胃散中枸橼酸含量的方法。用十八烷基键合硅胶柱分离枸橼酸,以0.5%磷酸二氢铵溶液(用磷酸调pH值至3.0)为流动相,检测波长210 nm。枸橼酸在1.0-15.0μg(r=0.999 9,n=6)范围内呈线性关系,线性方程为Y=67.88X+1.577(r=0.9999,n=6);平均回收率为97.75%,RSD为1.62%(n=6)。该方法简便、快速,可用于健胃散中枸橼酸含量测定。
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| 关 键 词： | 健胃散 枸橼酸 RP-HPLC |
Determination of citric acid in Jianwei San by RP-HPLC |
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| JIN Lu-sheng,LI Shu-gang,WANG Deng-lin,SHI Zi-xuan,ZHANG Hong,ZHANG Lei, WU Sheng-ping,ZHANG Xu.Determination of citric acid in Jianwei San by RP-HPLC[J].Journal of Traditional Chinese Veterinary Medicine,2012,31(2):42-44. |
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| Authors: | JIN Lu-sheng LI Shu-gang WANG Deng-lin SHI Zi-xuan ZHANG Hong ZHANG Lei WU Sheng-ping ZHANG Xu |
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| Institution: | (Gansu Institute of Veterinary Drug and Feed Inspection,Lanzhou Gansu 730030,China) |
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| Abstract: | To establish a RP-HPLC method for determination of citric acid in Jianwei San,a reverse-phase C18 column was used,the mobile phase was 0.5 mol/L ammonium biphosphate solution(adjusted the pH value to 3.0 with phosphoric acid)and the determination wave length was 210 nm.The results showed that the linear range of citric acid was 1.0-15.0 μg,the linear equation was Y=67.88X+1.577(r=0.999 9,n=6),and the average recovery was 97.75% with RSD of 1.62%(n=6).The method was simple,fast and suitable for the determination of citric acid in Jianwei San. |
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| Keywords: | Jianwei San citric acid RP-HPLC |
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