低温对燕山板栗枝条生理的影响研究

为了探讨低温处理对燕山板栗枝条生理的影响,以燕山板栗‘燕龙’品种一年生健壮枝条为材料,采取不同低温处理,测定其电导率、丙二醛(MDA)含量、多酚氧化酶(PPO)活性生理指标以及嫁接成活率。板栗枝条电解质渗出率、伤害率均随处理温度的降低而增加;在-20℃之前,MDA含量随温度的降低而增加,在-20℃,MDA含量达到最高,而在-25℃时MDA含量又降低到极低水平;PPO活性在0～20℃之间随温度的降低而逐渐降低,在-20℃之后PPO活性又升高;成活率随处理温度降低而降低,在0℃下的枝条嫁接成活率最高(60%)在-20℃以下处理的嫁接成活率为0%。-20℃是‘燕龙’板栗枝条的致死温度;MDA含量、PPO活性在其致死温度处出现拐点。初步认定在不同低温处理下,可以用MDA含量和PPO活性出现拐点的温度预示测定植物或材料的致死温度。

Influence of Low Temperature on the Physiological ofYanshan Chestnut Branches

In order to study the influence of low temperature on the physiological characteristics of‘Yanshan’ chestnut branches, the test materials were collected from one year robust branches of‘Yanlong’chestnut. In different low temperature conditions, conductivity, the content of MDA, the activity of PPO and the rate of graft were tested. The electrolyte leakage rate and the damage degree of chestnut branches were increasing with the temperature decreasing. Above -20℃ , the content of MDA was increasing with the temperature decreasing. At -20℃ , the content of MDA was highest; at -25℃ , the content of MDA was back to a very low level. Above -20℃, the activity of PPO was decreasing with the temperature decreasing, but under -20℃ the activity of PPO was increasing. The rate of survival was decreasing with the temperature decreasing. It had the highest rate of survival at 0℃ (60%). The rate of survival was 0% under -20℃ . -20℃ was the limit temperature of ‘Yanshan’chestnut branches. There were inflexion of the content of MDA and the activity of PPO at -20℃. It was confirmed preliminarily that used the temperature of the content of MDA and the activity of PPO to ensure the limit temperature of plant can bear.