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| 马传染性贫血病毒envgp90基因在实验感染马体内的遗传变异 | |
| 引用本文： | 王全凯,庞海,孔宪刚,宣华,章金刚,卢景良,费恩阁.马传染性贫血病毒envgp90基因在实验感染马体内的遗传变异[J].中国兽医学报,1999,19(3):207-211. |
| 作者姓名： | 王全凯 庞海 孔宪刚 宣华 章金刚 卢景良 费恩阁 |
| 作者单位： | 1. 解放军农牧大学兽医学院,长春,130062 2. 日本东京大学 3. 中国农业科学院哈尔滨兽医研究所 |
| 基金项目： | 日本国际协力财团资助,日本农林水产省家畜卫生试验场资助 |
| 摘 要： | 为确定马传染性贫血病毒（ＥＩＡＶ）ｅｎｇ ｇｐ９０基因的遗传变异特性，本研究应用ＥＬＡＶ日本分离强毒株Ｐ３３７－Ｖ７０实验感染了１匹健康马，经第２次感染后，实验马未呈现临床症状，ｇｐ９０基因的核苷酸序列被直接从这匹马的外周血和肝脏所获得的前病毒ＤＮＡ扩增。 |
| 关 键 词： | 马 传染性贫血病毒 gp90基因 前病毒 变异 遗传 |
Proviral Genomic Variation of EIAV Envelop gp 90 Gene Observed in Experimentally Infected Horse |
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| Wang Quankai,Pang Hai,Kong Xiangang,Xuan Hua,Zhang Jingang,Lu Jingliang,Fei Enge.Proviral Genomic Variation of EIAV Envelop gp 90 Gene Observed in Experimentally Infected Horse[J].Chinese Journal of Veterinary Science,1999,19(3):207-211. | |
| Authors: | Wang Quankai Pang Hai Kong Xiangang Xuan Hua Zhang Jingang Lu Jingliang Fei Enge |
| Abstract: | The present experiment was designed to observe the genetic variation of equine infectious anemia virus (EIAV) envelop gp 90 gene in infected horse. One horse was infected experimentally with P337V70 strain and showed no clinical signs after being infected at twice with the same virus strain. Seventeen proviral sequences covering principal neutralizing domain (PND) of EIAV gp 90 gene were obtained from the buffy coat and liver of the horse through PCR amplification and cloning. Comparative analysis of the sequences revealed that some sequences contained the nucleotide insertions in the PND region. The insertions might be generated by direct repeat and strand displacement of sequence segment in their PND gene, showing different lenghts. |
| Keywords: | equine infectious anemia virus gp90 gene provirus variation nucleotide sequence |
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