海湾扇贝边中氨基多糖的研究

本文对海湾扇贝边中氨基多糖进行了研究。海湾扇贝边经胃蛋白酶和胰匀浆水解去蛋白，继用乙醇沉淀得粗多糖。粗多糖用吸附法和透析法纯化，并经乙醇和ＣＴＡＢ进一步分级提纯，最终获得氨基多糖精制品。该品含有氨基己糖、己糖醛酸、硫酸基以及５种中性单糖。其琼脂糖凝胶电泳、高效液相色谱和红外光谱类于肝素表明，所得多糖为肝素样氨基多糖。

STUDIES ON GLYCOSAMINOGLYCANS FROM LEFTOVER BITS OF BAY SCALLOP

Glycosaminoglycans(GAG)from the leftover bits of Bay scallop(Argopectenirradians) was studieThe semifinished product of GAG was isolated from leftover bits byhydrolization of protein with pepsin and pancreas homogenate and pricipitation with alcohol.The final fraction of GAG was gained from the semifinished product through adsorptionwith activated charcoal , dialysis with dialyzer,and separation with alcohol and CTABsuccessively,The GAG of the final fraction contains hexosamine , hexosuronic acid andsulphate. The results of agarose gel electrophoresis , high performance iiquid chromato-graphy and infrared sepectrometry of the fraction were similar to that of heparin,Itindicated that the GAG of the fraction is heparin-like.