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藤本月季组织培养快繁研究
引用本文:李青,苏雪痕,李湛东. 藤本月季组织培养快繁研究[J]. 北京林业大学学报, 1999, 21(6): 17-21
作者姓名:李青  苏雪痕  李湛东
作者单位:北京林业大学园林学院,100083,北京
基金项目:国家引进国际先进农业科学技术计划(948计划) 
摘    要:该文是对最新引进的荷兰优良藤本月季进行组织培养快速繁殖研究.经过试验对比,筛选出适合多数品种增殖分化的培养基及生根培养基.最佳的继代增殖培养基为MS+6BA0.5~1.0mg·L-1,适宜的生根培养基为1/2MS+IBA0.2mg·L-1,生根率达100%.在试管苗的移栽试验中摸索了移栽基质等与成活率的关系,使移栽成活率达98%以上.

关 键 词:藤本月季  组织培养  MS培养基  快速繁殖  移栽

Rapid Propagation of Climbing Rose in vitro
Li Qing,Su Xuehen,Li Zhandong. Rapid Propagation of Climbing Rose in vitro[J]. Journal of Beijing Forestry University, 1999, 21(6): 17-21
Authors:Li Qing  Su Xuehen  Li Zhandong
Abstract:Rapid propagation of climbing rose from the Netherlands %in vitro% was studied.The optimal media of proliferating,rooting inducing culture for most climbing rose varieties from the Netherlands was selected. The best medium is MS medium supplemented with 6 BA 0.5~1.0 and NAA 0.01 mg5L+{-1}.Shoots were rooted on the 1/2?MS media supplemented with IBA 0.2 mg5L+{-1} and the rate of rooting was 100%.Suitable transplanting substrate and transplanting procedure are explored in transplanting plantlet experiment and transplanting survival rate was 98%.
Keywords:climbing rose   tissue culture   MS medium   rapid propagation   transplanting
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