Validation of a modified Petri-dish test to quantify aggressiveness of <Emphasis Type="Italic">Fusarium graminearum</Emphasis> in durum wheat |
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Authors: | W Purahong D Alkadri P Nipoti A Pisi M Lemmens A Prodi |
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Institution: | 1.Dipartimento di Scienze e Tecnologie Agroambientali,Alma Mater Studiorum Università di Bologna,Bologna,Italy;2.Department for Agrobiotechnology—IFA-Tulln, Institute of Biotechnology in Plant Production,BOKU—University of Natural Resources and Life Sciences,Tulln,Austria;3.Faculty of Agriculture and Life Science,Chandrakasem Rajabhat University,Bangkok,Thailand |
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Abstract: | Fusarium graminearum is a common agent causing Fusarium head blight (FHB) on wheat throughout the world. Aggressiveness is crucial for understanding
the interaction between host-pathogen in the FHB-wheat system. In this paper, we modified and validated the Petri-dish test
originally described by Mesterhazy (Phytopathologische Zeitschrift 93:12–25, 1978) to quantify the aggressiveness of 25 F. graminearum strains using four durum wheat cultivars with different resistance levels for FHB. The results were highly significant and
correlated with those obtained using adult plants in the growth chamber and in the field (r = 0.94, P < 0.001 and r = 0.65, P < 0.001, respectively). The Petri-dish test was further investigated for its repeatability and stability in different durum
wheat cultivars and highly significant correlation coefficients were obtained (r = 0.90–0.91 (P < 0.001), 0.89–0.95 (P < 0.001), respectively). In this study, we also demonstrated that germination rate reduction and coleoptile length reduction
are parameters involved with aggressiveness of F. graminearum. The mean of three disease parameters from the modified Petri-dish method is introduced in this paper as a new parameter
for aggressiveness and named “Petri-dish aggressiveness index”. The results obtained reveal that this modified Petri-dish
test is rapid, reliable and stable with different durum wheat cultivars, and yields highly significant correlation coefficients
with floret and ear inoculations, thus it is suitable to be used for quantification of aggressiveness of F. graminearum. |
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