| 基于转录组信息的艾纳香牻牛儿基焦磷酸合成酶基因(BbGPPS)的克隆及序列分析 |
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| 引用本文： | 官玲亮,夏奇峰,石小兵,蓝惠萍,陈振夏,赵致,庞玉新. 基于转录组信息的艾纳香牻牛儿基焦磷酸合成酶基因(BbGPPS)的克隆及序列分析[J]. 热带作物学报, 2016, 37(5): 901-909. DOI: 10.3969/j.issn.1000-2561.2016.05.009 |
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| 作者姓名： | 官玲亮 夏奇峰 石小兵 蓝惠萍 陈振夏 赵致 庞玉新 |
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| 作者单位： | 1. 中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室海南省艾纳香工程技术研究中心 海南儋州 571737;2. 中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室海南省艾纳香工程技术研究中心 海南儋州 571737;贵州大学生命科学学院贵州省药用植物繁育与种植重点实验室 贵州贵阳550025;3. 贵州大学生命科学学院贵州省药用植物繁育与种植重点实验室 贵州贵阳550025 |
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| 基金项目： | 国家自然科学基金（No. 81202910）；中央级公益性科研院所基本科研业务费专项资金（No. 1630032014015）；海南省中药现代化专项（No. ZY201410）。 |
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| 摘 要： | 采用RT-PCR和RACE(Rapid amplification of cDNA ends)技术,从艾纳香(Blumea balsamifera L.DC)的叶片中克隆到单萜化合物合成的关键酶牻牛儿基焦磷酸合成酶(BbGPPS)基因。研究结果显示,BbGPPS基因的cDNA全长1 692 bp,包含开放阅读框(ORF)1 083 bp,编码361个氨基酸;亚细胞结构定位于叶绿体,既非膜蛋白也非分泌性蛋白。疏水性分析显示,BbGPPS是亲水性蛋白。同源性比对结果显示,BbGPPS蛋白与其他植物中GPPS蛋白具有高度的相似性,且具有异戊烯基结构域。系统发育分析表明,所有序列被聚为5大类,BbGPPS与其他菊科植物聚类一类,与万寿菊(Tagetes erecta)TeGPPS亲缘关系最近,其次是甜菊(Stevia rebaudiana)SrGPPS。
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| 关 键 词： | 艾纳香 牻牛儿基焦磷酸合成酶 氨基酸序列 系统发育分析 |
Cloning and Analysis of Geranyl Pyrophosphate Synthase(GPPS) Sequence of Blumea balsamifera L.DC on Transcriptome Information |
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| GUAN Lingliang,XIA Qifeng,SHI Xiaobing,LAN Huiping,CHEN Zhenxi,ZHAO Zhi and PANG Yuxin. Cloning and Analysis of Geranyl Pyrophosphate Synthase(GPPS) Sequence of Blumea balsamifera L.DC on Transcriptome Information[J]. Chinese Journal of Tropical Crops, 2016, 37(5): 901-909. DOI: 10.3969/j.issn.1000-2561.2016.05.009 |
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| Authors: | GUAN Lingliang XIA Qifeng SHI Xiaobing LAN Huiping CHEN Zhenxi ZHAO Zhi PANG Yuxin |
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| Affiliation: | Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture / Hainan Provincial Engineering Research Center for;1 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture / Hainan Provincial Engineering Research Center fo;College of life Sciences, Guizhou University / Key Laboratory of Medicinal Plant Breeding and Planting in Guizhou Province,;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture / Hainan Provincial Engineering Research Center for;College of life Sciences, Guizhou University / Key Laboratory of Medicinal Plant Breeding and Planting in Guizhou Province,;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture / Hainan Provincial Engineering Research Center for;College of life Sciences, Guizhou University / Key Laboratory of Medicinal Plant Breeding and Planting in Guizhou Province, |
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| Abstract: | |
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| Keywords: | Blumea balsamifera L.DC Geranyl diphosphate synthase Amino acid sequence Phylogenetic analysis |
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