蝴蝶兰类原球茎玻璃化产生的原因及恢复效果研究

对蝴蝶兰(Phalaenopsis)组培生产中引起类原球茎玻璃化的原因以及玻璃化类原球茎再利用的效果进行了研究.结果表明,随着植物生长调节剂浓度增高和继代培养的时间延长,类原球茎玻璃化程度加重.当NAA浓度为5 mg/L、BA浓度为10 mg/L,继代培养到第9代时,2种培养基中玻璃化率分别高达71%和65%.玻璃化类原球茎的平均增殖率仅为2.2,再生植株率为183株/瓶,明显低于正常类原球茎的5.3和1 297株/瓶.玻璃化类原球茎在无植物生长调节剂培养基中经2～3代恢复培养后,数量下降到0.84%,玻璃化现象可得到明显恢复,恢复后的类原球茎的分化能力和植株生长状态与正常类原球茎一致,无变异现象发生,可继续应用于组培生产.

Study on the Cause and Restoration of Vitrification PLB in Tissue Culture and Rapid Propagation of Phalaenopsis

Studying on the causes and the recycle effects of vitrification protocorm in manufacture of Phalaenopsis.Results showed that:it aggravates the extent of protocorm vitrification with the increase of hormone concentration and the expansion of subculture time.The vitrification rates of two culture mediums rise to 71% and 65% respectively when the concentration of hormone NAA was 5mg/L,hormone BA was 10 mg/L and subculture the ninth generation.The reproduction ability and plant regeneration rate was lower distinctly than formal protocorm.The phenomenon of vitrification could restore significantly after culture 2 or 3 generations in the culture medium without hormone.The differentiation ability of protocorm restored and the state of plant growth was consistent with the formal protocorm.It could continue applying to the manufacture of tissue culture.