Identification and characterization of EST-SSRs in finger millet (<Emphasis Type="Italic">Eleusine coracana</Emphasis> (L.) Gaertn.) |
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Authors: | Bheema Lingeswara Reddy Inja Naga Lakshmi Narasu Mangamoori Sivaramakrishnan Subramanyam |
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Institution: | 1.Centre for Biotechnology,Jawaharlal Nehru Technological University (JNTUH),Hyderabad,India;2.Department of Agricultural Biotechnology,Acharya N.G. Ranga Agricultural University (ANGRAU),Rajendranager, Hyderabad,India |
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Abstract: | In recent years, microsatellites have become the most used markers for studying population genetic diversity. The increased
availability of the DNA sequences has given the possibility to develop EST-derived SSR markers. A total of 1,927 ESTs of Eleusine coracana available in the NCBI database were mined for SSRs. Di-nucleotides are the most occurring motifs accounting for more than
50% of the repeats, of which GA was the most abundant motif and tetra-nucleotides are the least occurring motifs. Of the 132
markers identified, 30 primer pairs based were synthesized. SSR markers were used for variety discrimination and genetic assessment
in 15 finger millet accessions; 20 primers showed polymorphism and 13 primers were identified as having a PIC value above
0.5. On the basis of the distribution of these polymorphic alleles, the 15 accessions were classified into two groups. This
study has demonstrated the potential of EST-derived SSR primer pairs in finger millet. These primers will serve as valuable
source for further breeding programs. |
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