| 梨S系矮化砧组培苗增殖系数和质量的影响因子 |
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| 引用本文： | 及华,张海新,赵玉芬,葛会波. 梨S系矮化砧组培苗增殖系数和质量的影响因子[J]. 河北农业科学, 2004, 8(2): 40-44 |
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| 作者姓名： | 及华 张海新 赵玉芬 葛会波 |
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| 作者单位： | 河北省农林科学院遗传生理研究所,河北,石家庄,050051;河北林业学校,河北,石家庄,050061;河北省林业科学院,河北,石家庄,050061;河北农业大学园艺学院,河北,保定,071000 |
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| 基金项目： | 河北省科技攻关项目 ( 972 45 5 0 6D) |
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| 摘 要： | 对梨S系矮化砧试管苗增殖系数和生长状态的影响因子进行了研究 ,结果表明 :S1 ～S6 不同砧木类型试管分化能力存在差异 ;培养基中添加 1 0mg/L的 6 BA ,既能保证试管苗较高的增殖率 ,又能保证试管苗的质量 ;试管苗再生能力随继代培养代数的增加而提高 ;培养基中GA3≤ 0 1mg/L和使用组培专用封口膜 ,可以有效提高试管苗的分化质量 ,防止玻璃苗产生。
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| 关 键 词： | 梨S系矮化砧 试管苗 增殖系数 继代培养 分化质量 |
| 文章编号： | 1008-1631(2004)02-0040-05 |
| 修稿时间： | 2004-03-22 |
Influencing Factors on Multiplication Coefficient and Regeneration Quality of Adventitious Shoots of the S Series Dwarfing Rootstock of Pear |
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| JI Hua,ZHANG Hai-xin,ZHAO Yu-fen,GE Hui-bo. Influencing Factors on Multiplication Coefficient and Regeneration Quality of Adventitious Shoots of the S Series Dwarfing Rootstock of Pear[J]. Journal of Hebei Agricultural Sciences, 2004, 8(2): 40-44 |
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| Authors: | JI Hua ZHANG Hai-xin ZHAO Yu-fen GE Hui-bo |
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| Affiliation: | JI Hua1,ZHANG Hai-xin2,ZHAO Yu-fen3,GE Hui-bo4 |
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| Abstract: | The influencing factors of multiplication coefficient and regeneration quality for adventitious shoots of the S series dwarfing rootstock of pear were studied. The results showed: The regeneration capability of the adventitious shoots in different rootstocks was different. The multiplication coefficient and the quality of the shoots were higher on culture media with 1.0 mg/L 6-BA. The more the subculture times were,the higher the regeneration capability was.keeping GA 3 concentration under 0.1 mg/L and use of appropriative sealing material could improve the quality of adventitious shoots and overcome vitrification. |
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| Keywords: | S series dwarfing rootstock of pear Adventitious shoots Multiplication coefficient Subculture Regeneration quality |
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