堆肥中产纤维素酶真菌的筛选及其产酶条件

采集牛粪及其堆肥样品,通过纤维素—刚果红平板培养基初筛和摇瓶发酵复筛得到1株分解纤维素能力较强的真菌菌株F12,经形态学初步鉴定为青霉属(Penillium)。对该株真菌进行了产纤维素酶的适宜碳源、氮源、初始pH、接种量、培养时间和温度等培养特性的研究,结果显示：F12菌株的适宜碳源为麸皮和微晶纤维素复合碳源,氮源为硫酸铵,最佳培养条件为：pH值在5～6之间,接种量为5%左右,培养时间120 h,培养温度30～35℃。在此条件下,该菌株的CMCase活性达到47.5 IU/mL,FPA活性达11.1 IU/mL。

Screening of the cellulase-producing fungi and its enzyme-producing conditions in compost

Cattle manure and its compost samples were selected to obtain an effective cellulose-decomposing microorganism, a fungi strain of F12 producing high level of cellulase was screened out by primary culture on plates of cellulose-Congo red medium and secondary fermentation in liquid medium. The strain was identified as Penicillium based on the morphologic taxonomy. The effects of carbon source, nitrogen source, initial pH of medium,inoculation quantity,culture temperature and time on cellulase activity produced by the strain were studied.The results indicated that the optimum conditions of producing cellulase were as follows: carbon source was bran and microcrystalline cellulose complex, nitrogen source was ammonium sulfate, initial pH value was 5-6,inoculation quantity was about 5%,culture temperature was 30-35℃,culture time was 120 h. Under these conditions,the activities of CMCase and FPA were 47.5 IU/mL and 11.1 IU/mL respectively．