The influence of cytokinins and ionic strength of Anderson's medium on shoot establishment and proliferation of evergreen azalea |
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Authors: | Chi-Ni Hsia Schuyler S. Korban |
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Affiliation: | (1) Perennial Plant Biogenetics Laboratory, Department of Natural Resources & Environmental Sciences, 310 Madigan Bldg., University of Illinois, 1201 W. Gregory, Urbana, IL, 61801, U.S.A |
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Abstract: | Shoot-tip explants of evergreen azalea cv. Fuchsia grown on Anderson's medium and containing different cytokinins produced the highest proliferation rate on a medium containing thidiazuron (TDZ). TDZ concentrations ranging from 0.23 to 2.3 M resulted in both good bud-break rate (4 to 5) and shoot quality (> 0.5 cm in length). Adding 2.3 M zeatin to Anderson's medium containing 0.23 or 2.3 M TDZ increased the number of axillary shoots/explant. However, increasing the zeatin concentration to 4.6 M resulted in a reduced shoot proliferation rate. A medium containing 1.15 M TDZ and 2.3 M zeatin resulted in an 18-fold increase for 'Fuchsia' and a 9-fold increase for 'Hino Crimson' after 6 weeks of culture. It was found that explants grown on a half-strength Anderson's medium with 87.6 mM sucrose generally had better shoot proliferation rate and shoot quality than at higher ionic strength. |
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Keywords: | Rhododendron evergreen azalea micropropagation thidiazuron |
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