| 辽中地区西瓜花叶病病原的分子鉴定 |
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| 引用本文： | 陈红运,赵文军,程毅,李明福,朱水芳.辽中地区西瓜花叶病病原的分子鉴定[J].植物病理学报,2006,36(4):306-309. |
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| 作者姓名： | 陈红运 赵文军 程毅 李明福 朱水芳 |
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| 作者单位： | 中国检验检疫科学研究院动植物检疫研究所, 北京 100029 |
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| 基金项目： | 国家质量监督检验检疫总局科研项目 |
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| 摘 要： | 利用ELISA和RT-PCR方法对采自我国辽中地区的西瓜花叶病样品进行检测,表明其病原为黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)。将此分离物(CGMMV-Wcn)的外壳蛋白基因克隆后进行序列分析,结果表明其cp基因由486个碱基组成,编码161个氨基酸,与已报道的其它分离物一致;CGMMV-Wcn所致症状与西瓜株系(CGMMV-W)相同,且二者cp基因的氨基酸序列完全一致,因此该分离物应为CGMMV-W。
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| 关 键 词： | 黄瓜绿斑驳花叶病毒 外壳蛋白基因 序列分析 |
| 文章编号： | 0412-0914(2006)04-0306-04 |
| 收稿时间： | 2005-08-24 |
| 修稿时间： | 2005年8月24日 |
Molecular identification of the virus causing watermelon mosaic disease in Mid-Liaoning |
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| CHEN Hong-yun,ZHAO Wen-jun,CHENG Yi,LI Ming-fu,ZHU Shui-fang.Molecular identification of the virus causing watermelon mosaic disease in Mid-Liaoning[J].Acta Phytopathologica Sinica,2006,36(4):306-309. |
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| Authors: | CHEN Hong-yun ZHAO Wen-jun CHENG Yi LI Ming-fu ZHU Shui-fang |
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| Institution: | Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China |
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| Abstract: | The virus sample collected from watermelon in MidLiaoning Province was detected with ELISA and RT-PCR.The result showed that the sample was infected by Cucumber green mottle mosaic virus.The coat protein gene of this isolate(CGMMV-Wcn) was sequenced.It comprised 486 nucleotides and encoded a putative protein of 161 amino acids.The cp amino acid sequence of CGMMV-Wcn was identical to that of CGMMV-W strain in Japan.The same symptoms on the watermelon and the sequence analysis indicated that CGMMV-Wcn should belong to CGMMV-W strain. |
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| Keywords: | Cucumber green mottle mosaic virus cp gene sequence analysis |
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