| 猪流行性腹泻病毒SHpd/2012株Nsp8基因分析及真核表达 |
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| 引用本文： | 丁思嘉,罗依然,周书亭,马世文,蒲良,杨亮,刘源平,蒋升瑶,叶力波利·达吾力,杨志彪.猪流行性腹泻病毒SHpd/2012株Nsp8基因分析及真核表达[J].上海交通大学学报(农业科学版),2019(2):64-69. |
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| 作者姓名： | 丁思嘉 罗依然 周书亭 马世文 蒲良 杨亮 刘源平 蒋升瑶 叶力波利·达吾力 杨志彪 |
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| 作者单位： | 上海交通大学农业与生物学院上海市兽医生物技术重点实验室 |
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| 基金项目： | 国家重点研发计划(2016YFD0500100);国家自然科学基金(31472211) |
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| 摘 要： | 应用生物信息工具和分子克隆技术对PEDV SHpd/2012株Nsp8基因编码的蛋白进行了结构和功能分析以及体外表达。结果显示,NSP8蛋白是一个含有194个氨基酸的亲水性蛋白,不含信号肽和跨膜区。SHpd/2012毒株高度保守的NSP8氨基酸序列与CV777、AJ1102等毒株同源性高。NSP8蛋白也是一个单体蛋白,共有5个优势抗原表位。此外,本研究成功地在293T细胞内表达了NSP8蛋白,为进一步研究猪流行性腹泻病毒NSP8蛋白的功能提供了理论基础。
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| 关 键 词： | 猪流行性腹泻病毒 Nsp8基因 真核表达 |
| 收稿时间： | 2018/8/7 0:00:00 |
Gene analysis and eukaryotic expression of Nsp8 in strain SHpd/2012 of porcine epidemic diarrhea virus |
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| DING Si-ji,LUO Yi-ran,ZHOU Shu-ting,MA Shi-wen,PU Liang,YANG Liang,LIU Yuan-ping,JIANG Sheng-yao,YELIBOLI Dawuli and YANG Zhi-biao.Gene analysis and eukaryotic expression of Nsp8 in strain SHpd/2012 of porcine epidemic diarrhea virus[J].Journal of Shanghai Jiaotong University (Agricultural Science),2019(2):64-69. |
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| Authors: | DING Si-ji LUO Yi-ran ZHOU Shu-ting MA Shi-wen PU Liang YANG Liang LIU Yuan-ping JIANG Sheng-yao YELIBOLI Dawuli and YANG Zhi-biao |
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| Institution: | Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China,Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China and Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology, Shanghai Jiaotong University,Shanghai 200240,China |
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| Abstract: | Bioinformatics tools were used to study the structure and function of NSP8 protein in strain SHpd/2012 of porcine epidemic diarrhea virus(PEDV).The results show that NSP8 is a hydrophilic protein,which contains 194 amino acids,without signal peptide and transmembrane region.Its conserved amino acid sequence is highly homologous to strains CV777 and AJ1102.Furthermore,the results also show that NSP8 is a monomeric protein with five dominant epitopes and can be successfully expressed in 293T cells,which provides a theoretical basis for further study of the function of PEDV NSP8. |
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| Keywords: | PEDV(porcine epidemic diarrhea virus) Nsp8 eukaryotic expression |
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