| 农杆菌介导黄瓜Tril基因转化及体系优化 |
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| 引用本文： | 肖婷婷,杜慧,潘健,孙敬贤,陈岳,张乐钰,潘俊松,王刚,何欢乐,蔡润.农杆菌介导黄瓜Tril基因转化及体系优化[J].上海交通大学学报(农业科学版),2019(3):17-22, 28. |
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| 作者姓名： | 肖婷婷 杜慧 潘健 孙敬贤 陈岳 张乐钰 潘俊松 王刚 何欢乐 蔡润 |
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| 作者单位： | 上海交通大学农业与生物学院 |
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| 基金项目： | 国家自然科学基金项目(31471156,3);上海市研究生教育创新研究计划(园艺学) |
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| 摘 要： | 采用农杆菌介导法将黄瓜表皮毛基因Tril转入黄瓜‘新泰密刺’品种,并研究了6-苄氨基腺嘌呤(6-BA)和卡那霉素(Kan)分别对再生和转化苗筛选效果的影响。结果表明,在黄瓜‘新泰密刺’转化中,Kan抗性苗出芽率为9.67%,生根率为3.67%,转化株阳性率为0.33%;分化培养基中6-BA适宜浓度为0.5 mg/L,出芽率最高(76.33%),平均出芽天数最短(19.66 d);生根培养基中Kan浓度为60 mg/L时筛选效果最好。3种筛选方式的研究表明,仅在生根培养基中添加Kan(60 mg/L)筛选,其转化周期最短(45 d),阳性率最高(达4.0%)。
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| 关 键 词： | 黄瓜 6-苄氨基腺嘌呤 卡那霉素 遗传转化 |
| 收稿时间： | 2018/11/14 0:00:00 |
Agrobacterium mediated transformation of CsTril and system optimization in cucumber(Cucumis sativus L.) |
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| XIAO Ting-ting,DU Hui,PAN Jian,SUN Jing-xian,CHEN Yue,ZHANG Le-yu,PAN Jun-song,WANG Gang,HE Huan-le and CAI Run.Agrobacterium mediated transformation of CsTril and system optimization in cucumber(Cucumis sativus L.)[J].Journal of Shanghai Jiaotong University (Agricultural Science),2019(3):17-22, 28. |
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| Authors: | XIAO Ting-ting DU Hui PAN Jian SUN Jing-xian CHEN Yue ZHANG Le-yu PAN Jun-song WANG Gang HE Huan-le and CAI Run |
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| Institution: | School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China and School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China |
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| Abstract: | |
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| Keywords: | cucumber 6-benzylaminopurine kanamycin genetic transformation |
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