Characterization of two monoclonal antibodies which recognize different subpopulations of chicken T lymphocytes |
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| Authors: | T Kondo M Hattori H Kodama M Onuma T Mikami |
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| Institution: | Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. |
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| Abstract: | Distribution among peripheral T lymphocyte subpopulations and biochemical properties of the chicken lymphocyte surface antigens defined by monoclonal antibodies (mAbs) Lc-4 and Lc-6 were examined. Two-color immunofluorescence analysis revealed that Lc-4 and Lc-6 antigens were expressed on mutually exclusive subpopulations of peripheral T lymphocytes but not on B lymphocytes. Lc-4 mAb precipitated a polypeptide with apparent molecular mass of 35 and 65 kilodalton under reducing and non-reducing conditions, respectively. These results indicated that the antigen recognized with Lc-4 was closely similar in tissue distribution and biochemical property to mammalian CD8 antigen. |
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