光状况对油松苗针叶及叶绿体的吸收光谱和室温荧光光谱性质的影响

本文对生长在红、绿、蓝光以及遮荫和阳生条件下的油松苗针叶及叶绿体的吸收光谱和室温荧光光谱的性质进行了研究。结果表明:遮荫和红光导致针叶增加对500nm～600nm光的吸收。它们的F_(735)激发光谱在500nm～600nm区有很高的荧光激发活性。针叶在室温下有两个荧光发射峰,F_(735)和F_(685)。它们的性质受光照条件的影响。阴生叶和红生叶的相对荧光强度最大。F_(735)/F_(685)比值阳生叶最小,红生叶最大。针叶、离休叶绿体、PSⅠ、PSⅡ及LHCP等在室温下都发射F_(735)和F_(685)两个荧光峰。并且,扑草净不仅使F_(685)相对荧光强度增大,同时也使F_(735)的相对荧光强度增加。

Studies on Effects of Light Regime on Absorption Spectrum and Fluorescence Spectrum of Chloroplast and Needle of Chinese Pine (Pinus tabulaeformis Carr.) Seedling.

Chinese pine seedlings were grown for 3 years in green house under different light conditions (under sun light, in shade, under red, blue,or green light). The effects on absorption spectrum and fluorescence spectrum of needle and cliloroplast were compared with each other. In shade or red light, the needle showed higher absorption at 500-600nm. The higher fluorescent intensity was shown by the F735 excited spectrum of such needles in the same region (500-600nm) . At room temperature the needle has two fluorescent emission peaks, i.e.F735 and F685. They are much effected by light regime. The relative intensity of fluorescence at room temperature is the highest in shade needle and red light needle.. Sun needle showed the owest F735/F685 ratio; and red light needle, the highest.This ratio is negatively related with accumulation of dry matter, and showed certain quantitative relationship with LHCP. At room temperature, needles, isolated chloroplast, PS1, PS2 and LHCP, all emitted F735 and F685 Peaks. Treatment of chlorrlast with prometryne not only increased the relative intensity of F685. but also that of F735 .Therefore; the significance of fluorescence at room temperature is rather complex, and should not be considered simply as a fluorescent activity of PSl and PS2