Involvement of Chromosomes 5A and 5D in Cold-Induced Abscisic Acid Accumulation in and Frost Tolerance of Wheat Calli |
| |
Authors: | G Galiba R Tuberosa G Kocsy J Sutka |
| |
Institution: | Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462 Martonvásar, Hungary;Istituto di Agronomia Generale e Coltivazioni Erbacee, Facoltàdi Agraria, Universitàdi Bologna, Via Filippo Re 6-8, 1-40126 Bologna, Italy. |
| |
Abstract: | The relationship between frost tolerance and abscisic acid (ABA) accumulation was studied in callus cultures of three wheat cultivars differing in the level of frost-tolerance, and of the 5A and 5D chromosome substitution lines from the frost-tolerant variety ‘Cheyenne’ into frost-sensitive ‘Chinese Spring’. Following cold hardening, the increase in ABA level in the calli of the two frost tolerant cultivars was significantly higher than in those of the frost-susceptible cultivar. Similarly, in 5A and 5D substitution lines, significantly higher ABA levels were detected than in the recipient ‘Chinese Spring’. One week-long ABA treatment at 26 °C induced a significantly higher level of frost tolerance than that achieved by cold hardening, irrespective of the frost sensitivity of the examined genotypes. |
| |
Keywords: | Triticum aestivum abscisic acid frost tolerance cold acclimatization callus culture |
|
|