| 快速检测柑桔黄龙病病原的研究 |
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| 引用本文： | 邓晓玲,梁志慧,唐伟文.快速检测柑桔黄龙病病原的研究[J].华南农业大学学报,1999,20(1):1-4. |
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| 作者姓名： | 邓晓玲 梁志慧 唐伟文 |
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| 作者单位： | 1. 华南农业大学资源环境学院,广州,510642
2. 华南农业大学食品科学系 |
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| 基金项目： | 国家自然科学基金,广东省自然科学基金 |
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| 摘 要： | 应用ＰＣＲ技术对柑桔黄龙病病原ＤＮＡ进行体外扩增，可建立一套快速，准确，有效的检测该病原的方法，研究结果表明：ＰＣＲ技术对柑桔黄龙病病原的检测具有很强的特异性，只有感染了黄龙病病原的样品，ＰＣＲ才呈阳性反应。文章报道了应用ＰＣＲ技术能检测已带病但尚未症状的柑桔黄龙病病株，该检测技术可以对柑桔黄龙病进行早期诊断，及是地控制带病苗木的传播，这对选育无病苗木及病害的综合治理具有很高的应用价值。
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| 关 键 词： | 柑桔 黄龙病 聚合酶链式反应(PCR) 检测 |
Studies on the Rapid Detection of Citrus Huanglongbing Pathogen |
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| Deng Xiaoling,Liang Zhihui,Tang Weiwen.Studies on the Rapid Detection of Citrus Huanglongbing Pathogen[J].Journal of South China Agricultural University,1999,20(1):1-4. |
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| Authors: | Deng Xiaoling Liang Zhihui Tang Weiwen |
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| Abstract: | The present study is aimed to develop an effective method for the detection of Liberobacter asiaticum that causes citrus Huanglongbing disease. It was shown that the DNA of the pathogen could be amplified by polymerase chain reaction (PCR) in vitro and the appearance of such DNA was significantly detected in the samples of infected citrus trees of the various cultivars. To use PCR for the detection of the infected trees without any symptom yet at the early stage of the infection. This technique can be effectively applied to screen disease free seedlings, establish disease free citrus nurseries, and control the spread of the diseased seedlings. |
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| Keywords: | citrus Huanglongbing polymerase chain reaction(PCR) detection |
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