通过花粉管途径将抗虫基因(CpTI)导入小麦的研究

利用带有ＣｐＴＩ基因的ｐＡＰＣｐＴＩ－１－Ｄ质粒通过花粉管途径的不同方法导入冬小麦９２（８）８０２，种子发芽经４０ｍｇ／Ｌ卡那霉素液筛选，得到１８株绿苗，移栽后成活１０株。提取这１０株小麦植株叶片的ＤＮＡ，经ＰＣＲ检测和ＰＣＲ－Ｓｏｕｔｈｅｒｎ杂交两次鉴定分析，证明去柱头滴加法中的５株小麦ＣｐＴＩ基因已整合到其基因组中，获得５株转基因９２（８）８０２小麦植株，长势良好，并得到饱满的种子。

Study on Introduction of Vermin-Resistance Gene(CpTI) into Wheat through Pollen-Tube Pathway Method

The plasmid(pAPCpTI-1-D) with CpTI gene was introduced into winter wheat 92(8)802 through pollen-tube pathway,eighteen green seedlings were obtained after screening the progeny in 40mg/L Kanamycin solution,and ten of them survived after transplanting.Results of PCR and Southern hybridization analysis proved that five plants treated by dropping DNA liquid after cutting off the stigma had integrated the CpTI gene into their genome.These five putative transgenic plants grew well and had full seed.