L.fendleri与B.napus原生质体融合改良野生优质工业用油植物研究

为了把野生优质工业用油植物基因导入到普通栽培油菜品种中 ,本文报道了野生优质工业用油植物L .fendleri的原生质体培养及其原生质体与普通栽培油菜品种原生质体融合。结果表明 :L .fendleri原生质体及两种植物融合原生质体在P8培养基上诱导出愈率高 ;来源于下胚轴的原生质体出愈率高于子叶原生质体 ;低剂量γ射线辐照处理更有利于促进原生质体融合 ;子叶原生质体间细胞融合频率高于下胚轴原生质体间细胞融合频率 ;B5培养基 +2mgZT L对提高融合原生质体细胞愈伤组织再分化最适宜 ;原生质体融合细胞再生植株高度 60～ 70cm ,叶片形状偏向L .fendleri,叶片大小、茎秆粗细、花药大小介于双亲之间 ,花型、花色偏于B .napus,花药空瘪 ,无花粉粒 ,自交不能结实

WILD INDUSTRIAL OIL PLANT SPECIES IMPROVED BY PROTOPLAST FUSION BETWEEN L.fendleri AND B.napus

The protoplast fusion between wild plant species L.fendleri of the genus Lesquerella and B.napus was studied in order to transfer the industrial oil gene in L.fendleri into B.napus. The results showed that the percentage of calli derived from protoplats isolated from cotyledon was lower than the that isolated from hypocotyls when the protoplats were cultured in P-8 medium.The percentage of protoplat fusion treated with γ|ray was higher than the that treated with PEG.The hybrid plant regeneration from fusion of protoplasts between L.fendleri and B.napus was the highest when the calli were cultured in B-5 medium+2mgZT/L.The height of hybrid plants was 60～70cm.The shape of leaves resembled more to those of L.fendleri; and the leaves were three times larger than L.fendleri's. The anther size and the stem height of the hybrid plants were intermediate between both parents.The flower type and colour were the same as B.napus. No seeds was obtained because no pollens produced in the anther.