树脂法绿色制备茶多酚研究

为了提高中低档茶叶资源利用率，解决目前茶多酚生产工艺使用较多氯仿、乙酸乙酯等有毒有机溶剂、易造成环境污染等问题，以中低档茶叶为原料，采用食用级乙醇和水2种易得、安全的溶剂，比较BYX、HPD200A、HZ103、HZ841、NKA-9、PA、ADS17、XDA5、XDA7、NPS1、NPS2、HP20等12种树脂对茶多酚、咖啡碱、儿茶素的静态吸附和解吸附效果，筛选出较好的绿色制备树脂BYX，并应用BYX树脂对茶多酚进行动态洗脱研究。结果表明，以2 BV/h流速、80%乙醇体积分数、3 BV洗脱体积的BYX树脂洗脱工艺参数进行茶多酚分离制备，茶多酚纯度和得率分别可达到98.36%和19.56%，咖啡碱含量为0.96%，儿茶素含量为80.82%，EGCG含量为46.52%。该茶多酚绿色制备法工艺简单易行，不使用乙酸乙酯、氯仿等有毒有机溶剂，整个工艺过程不产生毒害污染物，安全卫生，产品质量高，可为高质量茶多酚产品的工业化绿色生产制备提供新途径。

Preparation of the Tea Polyphenols by Resin Chromatography

The aim of this study is to improve the utilization rate of resources in cheap tea,and to solve the prolem of using chloroform,ethyl acetate and other toxic organic solvent in tea polyphenols production process,which is easy to cause environmental pollution.With cheap tea as the raw material,adopting the safe edible ethanol and water solvent,the effect of static adsorption and de-sorption on tea polyphenols,catechins,and caffeine by BYX,HPD200A,HZ103,HZ841,NKA-9,PA,ADS17,XDA5,XDA7,NPS1,NPS2,HP20 resin.The BYX macro porous resin was screened from the twelve kinds of resins.The BYX macro porous resin was then applied for dynamic elution of tea polyphenols.The result showed that in the condition of 1400 mL BYX resin column,37.5 g samples,2 BV/h flowing velocity,80% ethanol,3 BV elution volume,the purity and yield of tea polyphenols could respectively reach to 98.36% and 19.56%,and the content of catechins and EGCG could respectively reach to 80.82%and 46.52%,however the content of caffeine was only 0.96%.The green preparation method of tea polyphenols was wieldy,safety and health.Any toxic organic solvents and harm pollutants were not used in all process,such as ethyl acetate,chloroform.It may provide one new way to green manufacturing producing high quality tea polyphenols.