| 猪繁殖与呼吸综合征病毒GZ-KY1株的分离及ORF5基因的克隆分析 |
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| 引用本文： | 刘志杰,曾智勇,周莉,梁海英. 猪繁殖与呼吸综合征病毒GZ-KY1株的分离及ORF5基因的克隆分析[J]. 中国畜牧兽医, 2012, 39(12): 14-16 |
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| 作者姓名： | 刘志杰 曾智勇 周莉 梁海英 |
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| 作者单位： | 1. 贵州大学动物科学学院,贵州贵阳 550025;2. 贵州省动物疫病与兽医公共卫生重点实验室,贵州贵阳 550025;3. 贵州大学教学实验场,贵州贵阳 550025 |
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| 基金项目： | 贵州省农业科技攻关项目 |
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| 摘 要： | 本试验首次采用PK-15细胞,通过同步接种的方法,成功分离到了1株猪繁殖与呼吸综合征病毒(PRRSV,GZ-KY1株),并扩增克隆了该毒株的ORF5基因。试验结果表明,该毒株ORF5基因全长603 bp,系美洲型,与国内强毒株的氨基酸一致性高于弱毒株,与经典美洲型毒株及国内弱毒株亲缘关系较远。
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| 关 键 词： | PRRSV PK-15 Marc-145 分离鉴定 |
| 收稿时间： | 2012-06-08 |
Isolation and Clone Analysis of ORF5 Gene of PRRSV GZ-KY1 Strain |
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| LIU Zhi-jie , ZENG Zhi-yong , ZHOU Li , LIANG Hai-ying. Isolation and Clone Analysis of ORF5 Gene of PRRSV GZ-KY1 Strain[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(12): 14-16 |
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| Authors: | LIU Zhi-jie ZENG Zhi-yong ZHOU Li LIANG Hai-ying |
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| Affiliation: | 1. College of Animal Science,Guizhou University,Guiyang 550025,China;2. Guizhou Province Key Laboratory of Animal Epidemic Disease and Veterinary Public Health,Guiyang 550025,China;3. Teaching Experiment Farms,Guizhou University,Guiyang 550025,China |
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| Abstract: | In this experiment, a strain of porcine reproductive and respiratory syndrome virus (PRRSV) was isolated in PK-15 cells by using synchronous inoculation method, named GY-KY1 strain, and the ORF5 gene was cloned. The results demonstrated that GY-KY1 strain was American type and the ORF5 gene was 603 bp. The amino acid consistency of GY-KY1 strain with domestic virulent strain than the attenuated strain, and had the farest genetic relationship with the classic American strains and domestic attenuated strain. |
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| Keywords: | PRRSV PK-15 Marc-145 isolation and identification |
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