精原干细胞介导法制备转基因羊的研究进展

精原干细胞介导法制备转基因动物是用试验导入的方法将外源基因移入动物细胞并整合到其基因组中,伴随着精原干细胞分化成精子,通过受精最终使外源基因得以表达。近年来,随着对精原干细胞研究的不断深入,人们发现其在建立转基因动物方面具有巨大的应用潜力和优势。作者从精原干细胞的生物学特性及携带外源基因的原理等方面阐述了其应用于转基因动物制备的理论机制,同时介绍了精原干细胞介导法在制备转基因动物方面,特别是转基因羊生产中的应用现状。

Research Progress on Transgenic Sheep Produced by Spermatogonial Stem Cell-Mediated

Spermatogonial stem cell-mediated is to transfer the exogenous genes into the animal spermatogonia and the exogenous genes was integrated into its genome. With the original spermatogonial stem cells (SSCs) differentiation into sperm, the exogenous genes were expressed in their own offspring through fertilization. In recent years, people have realized its huge applications prospect and advantages in producing transgenic animals with the deepen research of SSCs. Then, the biological characteristics of SSCs as well as the principle of carrying exogenous gene into animal genome was described to clarify its theoretical mechanisms in preparation of transgenic animals, meanwhile, the research status of spermatogonial stem cell-mediated was introduced, especially for its application in the transgenic goat or sheep production.