利用微卫星标记分析蓝孔雀群体的遗传多样性*

 蓝孔雀不仅具有观赏价值,其作为特禽来养殖也具有较高的经济价值。为了进一步了解蓝孔雀群体的遗传变异状况,筛选出10对家鸡微卫星引物,利用PCR、聚丙烯酰胺凝胶电泳技术对西双版纳蓝孔雀群体的59个个体进行了遗传多样性检测。10个微卫星座位共检测到35个等位基因,各基因座位的等位基因数为2~5个,平均每个座位的等位基因数和有效等位基因数分别为3.50和3.00个;群体平均多态信息含量为0.5533,平均杂合度为0.6168。表明蓝孔雀群体遗传多样性丰富。

Genetic Diversity of Blue Peafowl Based on Microsatellite Markers

Blue peafowls as beautiful birds not only have the value of viewing and admiring, but also have high economic value when they are raised as a kind of special poultry. In order to evaluate its genetic variability and population structure further, 10 microsatellite loci screened from chicken were analyzed for a total of 59 individuals from Xishuangbanna, Yunnan province by using PCR and polyacrylaminde gel electrophoresis. A total of 35 alleles were detected across the 10 loci assayed. All the loci were polymorphic and the number of alleles varied from 2 to 5, giving a mean number of 3.50 alleles and a mean effective number of 3.00 alleles per locus. The average polymorphism information content and heterozygosity of 10 microsatellite loci were 0. 553 3 and 0. 616 8, respectively. The results indicated that Blue peafowl examined was abundant in genetic diversity.