| 橡胶树炭疽病菌的鉴定及rDNA-ITS序列分析 |
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| 引用本文： | 李继锋,刘先宝,蔡吉苗,林春花,黄贵修. 橡胶树炭疽病菌的鉴定及rDNA-ITS序列分析[J]. 中国农学通报, 2010, 26(12): 221-226 |
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| 作者姓名： | 李继锋 刘先宝 蔡吉苗 林春花 黄贵修 |
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| 作者单位： | 1. 海南大学环境与植物保护学院,海南儋州,571737;中国热带农业科学院环境与植物保护研究所海南省热带农业有害生物检测监控重点实验室,海南儋州,571737
2. 中国热带农业科学院环境与植物保护研究所海南省热带农业有害生物检测监控重点实验室,海南儋州,571737 |
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| 基金项目： | 热带特色经济作物重大病虫害防控技术研究与示范项目资助 |
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| 摘 要： | 摘要：从我国海南和云南橡胶主产区分离到22株橡胶炭疽病菌，生物学特性和形态学观察表明其中存在尖孢炭疽菌，ITS序列分析进一步证明侵染我国海南和云南橡胶的炭疽菌为胶孢炭疽和尖孢炭疽。ITS序列多样性分析表明，15株尖孢炭疽菌之间遗传差异较小，7株胶孢炭疽菌之间差异较大。
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| 关 键 词： | 水肥交互 水肥交互 南瓜 光合特性 产量 |
| 收稿时间： | 2010-01-04 |
| 修稿时间： | 2010-01-12 |
Identification and the internal transcribed spacer regions analysis of pathogen causing rubber anthracnose |
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| Li Jifeng,LiuXianbao,Cai Jimiao,Lin Chunhu,Huang Guixiu. Identification and the internal transcribed spacer regions analysis of pathogen causing rubber anthracnose[J]. Chinese Agricultural Science Bulletin, 2010, 26(12): 221-226 |
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| Authors: | Li Jifeng LiuXianbao Cai Jimiao Lin Chunhu Huang Guixiu |
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| Affiliation: | (1Environment and Plant Protection College, Hainan University, Danzhou Hainan 571737;2Key Laboratory for Baleful Biology Detection and Monitor of Tropical Agriculture of HainanProvince,Environment and Plant Protection Institute, CATAS, Danzhou Hainan 571737) |
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| Abstract: | Abstract: The internal transcribed spacer regions (5.8S/ITS) of the ribosomal RNA gene from 22 isolates of colletotrichum leaf disease of rubber were amplified through the polymerase chain reaction with the universal primers and sequenced. And their biological characters also tested. The result revealed that only 7 isolates belong to Colletotrichum gloeosporioides. The remaining 15 isolates represented Colletotrichum acutatum. Intraspecific genetic variation of Colletotrichum acutatum isolated from rubber was relatively low, which was found through rDNA-ITS sequence analyses. However, intraspecific genetic variation of Colletotrichum gloeosporioides was relatively abundant. |
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| Keywords: | rDNA-ITSzz |
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