Expression analysis of BsAPase14 acid phosphatase,a stress responsive boiling-stable protein from <Emphasis Type="Italic">Triticum aestivum</Emphasis> |
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| Authors: | Arun Dev Sharma Gurmeen Rakhra Jatinder Singh |
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| Institution: | 1.PG Department of Biotechnology,Lyallpur Khalsa College,Jallandhar,India;2.Dept of Molecular Biology and Biochemistry,Guru Nanak Dev University,Amrtisar,India |
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| Abstract: | Developmental regulation and effect of abiotic stresses (osmotic, salt, heat, and drought) on induction of boiling-stable
acid phosphatases (BsAPases) was studied in the growing wheat seedlings. SDS-PAGE revealed the induction of several boiling-stable
polypeptides in a spatial and temporal manner in the growing seedlings. Western blot analysis, using polyclonal acid phosphatase
antiserum, revealed the induction of a cross-reacting one 14 kDa boiling-stable acid phosphatase band (14 kDa, BsAPase14)
in endosperm at 7 and 14 days of germination (DOG). Various abiotic stresses (heat, salt, and osmotic) also elicit accumulation
of BsAPase14 in a tissue-dependent manner. Imposition of drought stress also resulted in an induction of boiling-stable protein
(BsAPase-14) which disappeared after removing the stress. Based upon these observations, the possible role of BsAPase14 in
water-stress tolerance is discussed. |
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