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| 家蚕病毒性软化病病毒抗血清的研制及应用 | |
| 引用本文： | 朱宏杰,赵新华,戴建一,吴纯清,王瀛,陆奇能,鲁兴萌.家蚕病毒性软化病病毒抗血清的研制及应用[J].蚕桑通报,2006,37(3):16-19. |
| 作者姓名： | 朱宏杰 赵新华 戴建一 吴纯清 王瀛 陆奇能 鲁兴萌 |
| 作者单位： | 1. 浙江大学,动物科学学院蚕蜂研究所,浙江,杭州,310029 2. 浙江省桐乡市蚕业公司,浙江,桐乡,314500 3. 浙江省桐乡市蚕业管理站,浙江,桐乡,314500 |
| 基金项目： | 浙江省科技厅重点科技项目(2003C22013) |
| 摘 要： | 从蚕区农户分离了一株家蚕病毒性软化病病毒,暂命名为BmIFV-CHN001。利用蔗糖密度梯度及差速离心法提纯该病毒,将提纯病毒免疫家兔3次,得到BmIFV抗血清。所制备的抗血清采用琼脂双扩散法检测养蚕农户家蚕样本,调查该病毒病在浙江蚕区的感染现状,调查显示农村饲养家蚕具有较高的感染率。 |
| 关 键 词： | 家蚕 病毒 软化病 抗血清 |
| 文章编号： | 0258-4069（2006）03-0016-04 |
| 收稿时间： | 2006-04-09 |
| 修稿时间： | 2006-04-09 |
Antiserum Preparation and Application of Infectious Viral Flacherie of Bombyx mori |
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| ZHU Hong-jie,ZHAO Xin-hua,DAI Jian-yi,WU Chun-qing,WANG Ying,LU Qi-neng,LU Xing-meng.Antiserum Preparation and Application of Infectious Viral Flacherie of Bombyx mori[J].Bulletin of Sericulture,2006,37(3):16-19. | |
| Authors: | ZHU Hong-jie ZHAO Xin-hua DAI Jian-yi WU Chun-qing WANG Ying LU Qi-neng LU Xing-meng |
| Institution: | 1. College of Animal Sciences, Zhejiang University, Hangzhou 310029, China; 2.Tongxiang Sericulture Corporation, Zhejiang Tongxiang 314500, China;3. Tongxiang Sericulture Supervision Station, Zhejiang Tongxiang 314500, China |
| Abstract: | Bombyx mori infectious flacherie virus was isolated in the laboratory from Tong-xiang city of Zhejiang province and named BmIFV-CHN001.The virions was purified by differential speed and sucrose.The purified virions immunized rabbit 3 times,getting the antiserum.The presence of BmIFV in Zhejiang was investigated with Gel Immunodiffusion test and the detections showed BmIFV are common in samples of Zhejiang. |
| Keywords: | Bombyx mori Virus Flacherie Antiserum |
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