|
|||||
|
|
| 不同产地猴头茵子实体粗多糖特征及其刺激巨噬细胞产生NO的活性 | |
| 引用本文： | 吴迪,张劲松,蒋俊,周帅,汪雯翰,刘艳芳,冯娜,唐庆九,罗珍,杨焱.不同产地猴头茵子实体粗多糖特征及其刺激巨噬细胞产生NO的活性[J].食用菌学报,2011,18(4):59-62. |
| 作者姓名： | 吴迪 张劲松 蒋俊 周帅 汪雯翰 刘艳芳 冯娜 唐庆九 罗珍 杨焱 |
| 作者单位： | 1. 农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201403 2. 南京农业大学生命科学学院,江苏南京210095;农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201403 3. 无限极(中国)有限公司,广东广州,510000 |
| 基金项目： | 上海市农委重点攻关项目[编号:沪农科攻字(2008)第8-2号]的部分研究内容 |
| 摘 要： | 提取7种不同来源的猴头菌(Hericium erinaceus)子实体粗多糖,对其理化性质和体外免疫活性进行分析.结果表明:不同产地的猴头菌子实体粗多糖含量和得率差异较大,以来源于黑龙江省牡丹江市海林县(H1)、林口县(H3)的子实体粗多糖含量、得率较高；各粗多糖提取物分子量分布相似,单糖组成均以葡萄糖为主,并含有岩藻糖和半乳糖；7种子实体粗多糖提取物均能增强小鼠巨噬细胞株RAW264.7生成NO的量,以500 μg/mL H3提取物活性最高、H1次之. |
| 关 键 词： | 猴头菌 粗多糖 一氧化氮释放 巨噬细胞 免疫调节 |
Partial Characterization of Polysaccharides from Seven Hericium erinaceus Fruit Body Samples and Their Effect on Nitric Oxide Release from Macrophages |
|
| WU Di , ZHANG Jingsong , JIANG Jun , ZHOU Shuai , WANG Wenhan , LIU Yanfang , FENG Na , TANG Qingjiu , LUO Zhen , YANG Yan.Partial Characterization of Polysaccharides from Seven Hericium erinaceus Fruit Body Samples and Their Effect on Nitric Oxide Release from Macrophages[J].Acta Edulis Fungi,2011,18(4):59-62. | |
| Authors: | WU Di ZHANG Jingsong JIANG Jun ZHOU Shuai WANG Wenhan LIU Yanfang FENG Na TANG Qingjiu LUO Zhen YANG Yan |
| Institution: | 1National Engineering Research Center of Edible Fungi;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China; 2College of Life Sciences,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China; 3Infinitus(China) Company Ltd,Guangzhou,Guangdong 510000,China |
| Abstract: | Crude polysaccharide preparations were prepared by hot(100 ℃) water extraction and ethanol precipitation from Hericium erinaceus fruit bodies artificially cultivated at seven different facilities located in Fujian,Zhejiang and Heilongjiang Provinces of China.Highest yields of crude extract containing the highest amounts of polysaccharide were obtained from fruit body samples(H1 and H3) grown in Heilongjiang.HPLC analysis revealed that crude polysaccharide material extracted from the seven fruit body samples had similar molecular weight distributions,and all contained relatively high amounts of glucose together with smaller quantities of fucose and galactose.All the test samples exhibited immunomodulatory activity,assessed on the basis of nitric oxide production by RAW 264.7 macrophages,with highest NO levels recorded when the cells were exposed to 500 μg/mL of material extracted from H3 and H1 fruit bodies. |
| Keywords: | Hericium erinaceus polysaccharide nitric oxide production macrophages immunoregulation |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |