| 桉树无性系与亲本及桉属其它种遗传关系分析 |
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| 引用本文： | 刘海龙,王以红,吴幼媚,蔡玲,覃子海,黄金使.桉树无性系与亲本及桉属其它种遗传关系分析[J].广西林业科学,2011,40(1):4-7. |
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| 作者姓名： | 刘海龙 王以红 吴幼媚 蔡玲 覃子海 黄金使 |
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| 作者单位： | 广西林业科学研究院,南宁,530002 |
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| 基金项目： | 广西壮族自治区科技厅项目 |
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| 摘 要： | 对桉属(Eucalyptus)的11个种和3个元性系的ITS序列进行了比对分析,桉属的不同种以及3个无性系的ITS长度范围为633～678 bp.分析表明,3个无性系的ITS为假基因,其ITS序列长度比近缘种长38～45 bp.因此,通过ITS引物扩增和测序可以将无性系与其它种类、品种或类型区分.系统发育分析表明,柠檬...
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| 关 键 词： | 桉属 无性系 鉴定 ITS序列 遗传关系 |
Analysis of Genetic Relationship among Eucalyptus Clones,Parents,and Other Species in Eucalyptus |
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| LIU Hai-long,WANG Yi-hong,WU You-mei,CAI Ling,QIN Zi-hai,HUANG Jin-shi.Analysis of Genetic Relationship among Eucalyptus Clones,Parents,and Other Species in Eucalyptus[J].Guangxi Forestry Science,2011,40(1):4-7. |
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| Authors: | LIU Hai-long WANG Yi-hong WU You-mei CAI Ling QIN Zi-hai HUANG Jin-shi |
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| Institution: | (Guangxi Forestry Research Institute,Nanning 530002,China) |
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| Abstract: | Sequences of the internal transcribed spacer(ITS) region of the nuclear ribosomal DNA were obtained from 11 species of Eucalyptus and 3 clones,ranging from 633 bp to 678 bp in size.ITS analysis indicated that the non-functional pseudogenes were identified in 3 clones.Compared with other species,the amplified fragments of 3 clones were 38～45 bp longer than those of 11 Eucalyptus species.Therefore,we could differentiate the clones from other species in Eucalyptus by amplifying and sequencing of ITS primers.Phylogenetic analysis revealed that E.pellita,E.urophylla and E.citriodora in genetic relationship were near,and E.grandis was related to E.exserta.These related species in Eucalyptus could be used as parental combinations of hybridization in order to combine desirable characteristics from different Eucalyptus species. |
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| Keywords: | Eucalyptus clone identification ITS sequence genetic relationship |
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