| 富有柿果ACC氧化酶cDNA的克隆及其序列分析 |
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| 引用本文： | 张丽,唐霞,马俊莲,刘月英.富有柿果ACC氧化酶cDNA的克隆及其序列分析[J].华北农学报,2007,22(1):69-72. |
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| 作者姓名： | 张丽 唐霞 马俊莲 刘月英 |
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| 作者单位： | 1. 河北农业大学,食品科技学院,河北,保定,071001
2. 河北经贸大学,生物工程系,河北,石家庄,050061 |
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| 基金项目： | 河北省国际科技合作攻关项目(04395501D-3) |
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| 摘 要： | 以富有柿果为材料,用RNA提取试剂盒提取总RNA,根据已报道的柿果ACC氧化酶(1-aminocyclopropane-l-carboxylic acid oxidase,ACO)基因的序列设计并合成1对引物,通过RT-PCR方法获得1条约1.2 kb特异片段,把该片段连接到pGEM-T easy vector上进行测序,其全长共1 237 bp,编码区697 bp,共编码231个氨基酸残基。序列分析结果表明,该序列与DK-ACO1的cDNA序列同源性达99%,氨基酸的同源性达98%。
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| 关 键 词： | 柿果 ACC氧化酶 RT-PCR 序列分析 |
| 文章编号： | 1000-7091（2007）01-0069-04 |
| 修稿时间： | 2006-12-08 |
Cloning and Sequence Analysis of a cDNA Encoding ACC Oxidase from Fuyu Persimmon |
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| ZHANG Li,TANG Xia,MA Jun-lian,LIU Yue-ying.Cloning and Sequence Analysis of a cDNA Encoding ACC Oxidase from Fuyu Persimmon[J].Acta Agriculturae Boreali-Sinica,2007,22(1):69-72. |
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| Authors: | ZHANG Li TANG Xia MA Jun-lian LIU Yue-ying |
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| Institution: | 1. College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China; 2. Biological Science and Engineering College, Hebei University of Ecnomic and Business, Shijiazhuang 050061, China |
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| Abstract: | Total RNA was extracted from Fuyu persimmon fruits and reverse transcribed to first strand of eDNA. A pair of primers were designed and synthesized based on the sequence reported. The specific PCR product of 1-aminocyclopropane-1-carboxylic acid oxidase was obtained using the first strand of eDNA as template. The product was ligased to pGEM^8 -T easy vector and sequenced. The sequencing data showed that the PCR product was 1 237 bp with 697 bp of coding region, encoding 231 predicted amino acid residues. Comparison with the eDNA sequence from DK-ACO1 indicated the homology was 99 %, and amino acid identities were 98 %. |
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| Keywords: | Persimmon fruit Fuyu ACC oxidase RT-PCR Sequence analysis |
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