黄连汤有效部位对鸭疫里氏杆菌的抑菌率

测定了黄连汤有效部位对鸭疫里氏杆菌Riemerella anatipestifer的抑菌率。将组成黄连汤的黄连Coptis chinensis，黄柏Phellodendrion amurense和甘草Glycyrrhiza uralensis 3味中草药按规定比例混合，采用水提醇沉法提取有效部位，制成含生药为229.9 g·L^－1 的黄连汤水提醇沉浓缩物。首先测定鸭疫里氏杆菌生长的标准质量浓度曲线，其次在不同药物质量浓度下分别加入振荡培养12 h的鸭疫里氏杆菌 20，50，120，190和250 μL，测定鸭疫里氏杆菌的吸光度。根据标准曲线关系式，计算出药物作用后细菌的浓度，进而得出各种质量浓度下药物对细菌的抑菌率，并对抑菌率进行方差分析。结果表明，当药物质量浓度是38.0 g·L^－1，菌量为20和50 μL时，对应的显著性抑菌率为99.99%和99.98%；药物质量浓度为46.0 g·L^－1，菌量为120 ，190和250 μL 时，对应的显著性抑菌率分别为99.99%，99.98%和99.98%。抑菌率都在99.98%以上。用紫外分光法研究药物抑菌率，准确、方便、重复性好；药物最优抑菌的临界浓度为29.0 g·L^－1。图1表3参14

Inhibitory rate of a coptidis rhizoma decoction against Riemerella anatipestifer bacteria

To determine the inhibitory rates for effective ingredients of a coptidis rhizoma（Coptis chinensis） decoction against the bacteria Riemerella anatipestifer and to further the study of drugs acting on the outer membrane of a bacterial protein,extractions were made from a coptidis rhizoma decoction made of coptidis rhizoma,cortex phellodendri（Phellodendron amurense）,and licorice root（Glycyrrhiza uralensis） using water extraction and alcohol precipitation methods.First,a standard concentration curve for R.anatipestifer was used.Next,bacteria of 250,190,120,50 and 20 μL were added to drug concentrations of 115.0,77.0,57.0,46.0,38.0,33.0 and 29.0 g·L-1 with three replications.Then,after oscillating the culture for 12 h,the optical density（OD） values were measured.Afterward,the bacterial content for each drug concentration was calculated,the bacterial inhibitory rate was determined,and an analysis of variance was conducted on the inhibition rate.Results showed that for a drug concentration of 38.0 g·L-1 and 20 μL of R.anatipestifer,the antibacterial rate was 99.99%,whereas with 50 μL the rate was 99.98%.However,for 46.0 g·L-1,the antibacterial rate against R.anatipestifer with 120 μL was 99.99%,with 190 μL it was 99.98%,and with 250 μL it was 99.98%.The optimal and critical drug concentration for antibacterial action was 29.0 g·L-1.It showed a strong antibacterial effect for this medicine indicating further research would be beneficial.