Klyveromyces lactis酵母生产乳糖酶发酵条件的研究

为了获得一株产乳糖酶乳酸克鲁维酵母(Kluyveromyces lactis ATCC 12426)的最佳发酵参数，通过正交试验优化了产乳糖酶的培养条件。结果表明：最佳种子培养基组分为：葡萄糖2%、酵母膏0.35%、蛋白胨0.35%。发酵培养基的最佳组分为：乳糖4%、葡萄糖2%、酵母膏0.18%、尿素0.15%、KH2PO4 1%、MgCl2 0.1%、MnCl2 0.01%。最佳发酵条件为：接种量5%，温度30℃，初始pH 7.0，摇床转速140 r/min，培养时间18 h。最佳条件下摇瓶发酵其胞内酶活高达20.861 U/mL。

Study on the Fermentation Conditions of the Yeast Kluyveromyces lactis Producing Lactase

To achieve the optimal fermentation conditions of the lactase-producing yeast Kluyveromyces laces, the optimal cultural conditions were obtained using orthogonal design. The optimal seed medium compositions were as follows： glucose 2%, yeast extract 0.35%, peptone 0.35%. Meanwhile, the optimal compositions of fermentation medium were as follows： lactose 4%, glucose 2%, urea 0.15%, KH2PO4 1%, MgCl2 0.1%, MnCl2 0.01%. The cultural conditions were： inoculation size of 5%, at a temperature of 30℃, initial pH 7.0, shakes flask rotation of 140 r/min and culture time of 18 h. Under these conditions, the lactase activity of K. lactis could reach 20.861 U/mL.