中国西门塔尔太行类群牛肉质性状与SLC27A1基因多态性关联分析

为探索SLC27A1基因对牛肉质性状的影响,选取中国西门塔尔牛太行类群牛公牛、阉牛各20头为研究对象,采用PCR法分析SLC27A1的基因遗传多态性。检测到SNP位点为S5650:TC,该位点存在有3种基因型,其中CC基因型频率较高(62.5%),TT基因型频率最低(12.5%)。采用GLM对肉质相关性状与SLC27A1基因进行关联分析,结果表明,剪切力、肉色a、肉色b表型中,CC与TT及CT与TT差异均极显著(P0.01),其他性状间差异不显著(P0.05),阉牛与公牛间对肉质的弹性与咀嚼性差异显著(P0.05)。采用Real-time PCR分析,CC基因型在肌肉与脂肪中的表达量比基因型CT与TT差异显著(P0.05),阉牛与公牛之间的表达量在肌肉中差异极显著(P0.01)。SLC27A1基因S5650:TC位点突变对中国西门塔尔牛太行类群肉质性状存在一定的遗传效应。

Correlation Analysis on Polymorphism of SLC27A1 Gene and Its Relationships with Meat Quality Trait in Chinese Simmental Taihang Group

20 bulls and bullocks were chosen respectively as the research object in Chinese Simmental Taihang group to ex-plore SLC27A 1 genetic influence on beef quality traits. Genetic polymorphism of SLC27A 1 was analyzed by PCR. One T→C mutation was detected at 5650 bp of SLC27A 1 gene. There are three kinds of genotype in the loci, CC, TT and CT. And the CC genotype frequency was higher (62.5%), TT genotype frequency lowest (12.5%). The relationships between the geno-types and meat quality traits were analyzed by GLM. The results showed the difference was significant (P<0.01) between genotype CC and TT, CT and TT in the phenotypes of shear force, color a and color b. The difference in elasticity and the chewiness was significant (P<0.01) between bullocks and bulls. The Real-time PCR was used to study the expression of dif-ferent genotype in tissues. The results showed that the expression difference between CC genotype and CT and TT genotype was significant in muscle and fat for SLC27A1(P<0.05). And the difference was extremely significant between bullocks and bulls in the muscles (P<0.01). This study proves that S5650:T > C locus mutation of the SLC27A1 gene maybe have some ge-netic effect on meat trait in Chinese Simmental TaiHang group.