复合酶辅助微波技术提取红枣多糖的工艺研究

采用复合酶辅助微波技术提取红枣(Ziziphus jujuba Mill.)中的多糖。结果表明,复合酶解的最佳工艺为木瓜蛋白酶添加量占原料干物质重量的0.6%,果胶酶添加量占原料干物质重量的1.0%,置于恒温45℃条件下,酶解90 min;微波提取最佳工艺为以蒸馏水作为提取溶剂,微波进料泵频率12 Hz,提取功率36 k W,物料比(m∶m)为1∶8,提取2次,红枣多糖的提取率可达5.24%。与其他提取方法相比,该方法高效、环保、选择性高。

Study on Extraction of Polysaccharide from Red Date by Multiple Enzymatic Assisted Microwave

Multiple enzymatic assisted microwave technology was used to extract red date (Ziziphus jujuba Mill.) polysaccha-ride. The results showed that the optimum method of multiple enzymatic were as follows:consumption of the papain as dry raw material matter weight of 0.6%,and pectinase dosage of dry raw material matter was 1.0%,and the extraction temperature was 45℃for 90 min. The optimum condition for microwave extraction was distilled water as extraction solvent, feeding pump frequency 12 Hz,the microwave power 36 kW, the proportion of raw material to solvent 1:8 twice (m:m).In the condition,the yield of polysaccharide was 5.24%. Compared with other methods,the method was high efficient, environmental and selective.