利用PCR技术鉴别畜禽肉中禽源性成分研究

为了建立一种快速、特异的禽源性成分检测方法,以线粒体16S r RNA基因序列为靶位点设计鸡、鸽、鹌鹑特异性引物,以常见畜禽肉(包括羊肉、牛肉、猪肉、兔肉、鸽肉、鹌鹑肉、鸡肉、鸭肉、鹅肉等)DNA为模板,进行PCR扩增和特异性检测。结果表明,筛选的引物能够有效地对动物源性成分进行检测,方便简洁,可快速鉴别畜禽肉食品中含有的鸡源性、鸽源性、鹌鹑源性成分。

Studies on the Identification of Poultry Origin Ingredients in Livestock and Poultry Meat by PCR Technique

In order to establish a quick and specific peculiar method, which could identify the components of poultry origin. 16S rRNA gene sequence was use as target site, the specific primers of chicken, pigeon meat and quail meat were designed. The DNA of common livestock and poultry meat including mutton, beef, pork, rabbit meat, pigeon meat, quail meat, chicken, duck and goose were used as template. Though PCR amplification and specific detection, a quick determination method was established to identify the components of poultry origin. The results showed that the selected primer could identify the components of animal origin effectively and quickly. The method was convenient and concise, and could detect the chick-en origin, pigeon origin and quail origin in poultry food quickly and accurately.