木通属植物种质资源的SRAP分析 |
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引用本文: | 黄佩蓓,陈世华,王飞,曾贤.木通属植物种质资源的SRAP分析[J].湖北农业科学,2016(7):1747-1750. |
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作者姓名: | 黄佩蓓 陈世华 王飞 曾贤 |
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作者单位: | 江西中医药大学基础医学学院,南昌,330004 |
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基金项目: | 江西省科技厅科技支撑计划项目(2009BSB0940) |
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摘 要: | 采用SRAP标记技术,对木通属(Akebia Decne.)25份药用植物材料进行遗传多样性分析。结果表明,在选用的12对引物中,共检测到173条多态性条带,多态性比率达83.2%,相似系数变化范围为0.275 8~0.931 0,UPGMA聚类分析将25个供试样品分为二类:五叶木通为一类,白木通和三叶木通聚为一类;SRAP标记适合木通属植物的遗传多样性、亲缘关系分析。
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关 键 词: | 木通属(Akebia Decne.) 相关序列扩增多态性(SRAP) 遗传多样性 |
SRAP Analysis on Akebia Decne. Germplasm Resources |
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Abstract: | Genetic diversity of 25 materials collected from three species of A kebia Decne. were detected by sequence-related amplified polymorphism(SRAP) molecular marker. The results showed that, in 12 pairs of primers, 173 polymorphism bands were detected, percentage of polymorphic bands was 83.2%, and the genetic similarties ranged from 0.2758 to 0.9310. The 25 materials were clustered into 2 groups by UPGMA. Akebia quinata Decne. formed a single cluster and A. trifoliata var. australis and A . trifoliate Koidz formed another cluster. The results indicated that SRAP markers are suited for the analysis of genetic diversity and relationship of A . Decne.. |
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Keywords: | A kebia Decne sequence-related amplified polymorphism(SRAP) genetic diversity |
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