木薯MeP5CS和MeP5CR基因克隆及其干旱胁迫下的表达分析

在不同浓度(0、20%、30%、40%、50%)PEG处理下考察木薯(Manihot esculenta Crantz)叶片中脯氨酸含量的动态变化。从木薯中分别克隆了Me P5CS和Me P5CR基因,对其序列进行生物信息学分析,并分析了Me P5CS和Me P5CR基因在20%PEG胁迫处理下各组织中的表达。结果表明,脯氨酸含量受渗透胁迫快速诱导,在一定范围内随PEG浓度升高而明显升高,表现出较好的相关性。序列分析表明,Me P5CS基因c DNA全长2 217 bp,编码738个氨基酸,含有P5CS保守结构域;Me P5CR基因c DNA全长828 bp,编码275个氨基酸,含有P5CR保守结构域,它们分别与麻风树和蓖麻中的P5CS、P5CR亲缘关系较近。Me P5CS和Me P5CR基因在转录水平受到渗透胁迫的调控。

Gene Cloning of MeP5CS and MeP5CR in Cassava and Their Expression Analysis under Drought Stress

Dynamic changes of proline content were investigated in cassava (Manihot esculenta Crantz) leaves in response to different concentration of PEG (polyethylene glycol) treatments (0,20℅,30℅,40℅ and 50℅). MeP5CS and MeP5CR were cloned in cassava and their sequences were analysed by bioinformatics method. The expression of MeP5CS and MeP5CR genes in different organs was analysed under 20℅ PEG treatment. The results showed that proline content were rapidly induced by osmotic stress,and at a certain extent,the increase of proline content was linearly correlated with PEG concentrations. Sequence analysis revealed that full length cDNA sequence of MeP5CS was 2 217 bp,which encoded 738 amino acids and contained P5CS conserved domain. The full length cDNA sequence of MeP5CR was 828 bp,which encoded 275 amino acids and contained P5CR conserved domain. They both had close genetic relationship with P5CS and P5CR,respectively,in Jatropha curcas and Ricinus communis. qRT-PCR results showed that the expression of both MeP5CS and MeP5CR were regulated by osmotic stress.