| 抗苯磺隆猪殃殃乙酰乳酸合成酶的突变研究 |
| |
| 引用本文： | 孙健,王金信,张宏军,刘君良,卞圣楠. 抗苯磺隆猪殃殃乙酰乳酸合成酶的突变研究[J]. 中国农业科学, 2010, 43(5): 972-977. DOI: 10.3864/j.issn.0578-1752.2010.05.011 |
| |
| 作者姓名： | 孙健 王金信 张宏军 刘君良 卞圣楠 |
| |
| 作者单位： | （山东农业大学植物保护学院）; |
| |
| 基金项目： | 国家“十一五”科技支撑计划(2006BAD08A09) |
| |
| 摘 要： | [目的]近几年,中国大部分冬小麦田猪殃殃(Galium agarine L.)用苯磺隆已无法有效控制.为明确猪殃殃对苯磺隆产生抗药性的分子机制,本试验从分子水平上开展研究,以初步明确乙酰乳酸合成酶(ALS)氨基酸序列的突变位点.[方法]通过对敏感和抗药性猪殃殃生物型ALS基因片段进行扩增、克隆和测序,比对2种生物型的ALS序列.[结果]与敏感生物型猪殃殃的ALS相比,抗药性猪殃殃生物型ALS有3个位点发生突变,其中位于高度保守区Domain B的第574位色氨酸被甘氨酸所取代.[结论]该位点的突变可能是猪殃殃对苯磺隆产生抗药性的主要原因.
|
| 关 键 词： | 抗药性 猪殃殃 乙酰乳酸合成酶 基因突变 |
| 收稿时间： | 2009-08-24; |
Study on Mutations in ALS of Resistance to Tribenuron-Methyl in Galium aparine L. |
| |
| SUN Jian,WANG Jin-xin,ZHANG Hong-jun,LIU Jun-liang,BIAN Sheng-nan. Study on Mutations in ALS of Resistance to Tribenuron-Methyl in Galium aparine L.[J]. Scientia Agricultura Sinica, 2010, 43(5): 972-977. DOI: 10.3864/j.issn.0578-1752.2010.05.011 |
| |
| Authors: | SUN Jian WANG Jin-xin ZHANG Hong-jun LIU Jun-liang BIAN Sheng-nan |
| |
| Affiliation: | SUN Jian1,WANG Jin-xin1,ZHANG Hong-jun2,LIU Jun-liang1,BIAN Sheng-nan1 (1College of Plant Protection,Sh,ong Agricultural University,Tai'an 271018,Sh,ong,2Institute for the Control of Agrochemicals,Ministry of Agriculture,Beijing 100125) |
| |
| Abstract: | Objective In recent years,Galium aparine L.in most winter wheat fields in China could not be controlled by tribenuron-methyl.The objective of this study is to understand the molecular basis of the resistance mechanism to tribenuron-methyl in G.aparine and to find the specific mutation sites in amino acid sequence of acetolactate synthase (ALS) in the resistant biotype of G.aparine.Method Fragments encoding the ALS were amplified and cloned from G.aparine,susceptible (S) and resistant (R) biotypes to tribenu... |
| |
| Keywords: | resistance Galium aparine L. acetolactate synthase gene mutation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
|
点击此处可从《中国农业科学》下载全文 |
|
