猪繁殖与呼吸综合征病毒核衣壳蛋白与糖蛋白的表达纯化及鉴定

Expression，Purification and Identification of Nucleocapsid Protein and Glycoprotein of Porcine Reproductive and Respiratory Syndrome Virus

In order to gain nucleocapsid protein （N） and glycoprotein （E） of porcine reproductive and respiratory syndrome virus （PRRSV）， the total RNA was extracted，ORF7 and ORF5 genes were obtained by RT-PCR， inserted into pET-28a(+) vector， and then transformed into Escherichia coli BL21（DE3），respectively. The expressed products were identified by SDS-PAGE and Western blotting， and purified by affinity chromatography. The results showed that N and E fusion protein were successfully expressed and the proteins had good reactionogenicities by Western blotting analysis. The purities of the purified proteins were 89% and 90%, respectively. This study could lay foundations for molecular biological function research and establishment of test methods for detection antibodies of PRRSV.