鹿药提取物清除羟基自由基的研究

采用浸提、索氏提取、大孔吸附树脂分离等技术制备鹿药提取物,包括:鹿药水提液、醇提液、醇提水溶液,醇提液浓缩后经大孔树脂柱水洗后依次用φ为30%、50%、70%、90%的乙醇洗脱分离得到Ⅰ、Ⅱ、Ⅲ、Ⅳ4部分干物质,从Ⅲ中分离得到5个黄酮类化合物:3-甲氧基-8-甲基槲皮素(A)、8-甲基木犀草素(B)、3′-甲氧基木犀草素(C)、木犀草素(D)和槲皮素(E).采用邻二氮菲-Fe2+-H2O2氧化法测定鹿药提取物抗氧化清除羟基自由基(HO.)的能力,结果表明,在试验浓度范围内,除鹿药醇提液之外,其他提取物制备液都具有清除HO.的作用,清除率23.30%~98.07%,其中5个黄酮单体化合物清除HO.的能力较强,且随浓度增大清除作用增强,并且作用强度明显好于对照维生素C.可见鹿药中具有较好的抗氧化活性成分.

Study on the Scavenging Hydroxyl Radical of the Extracts from Smilacina japonica

The extractions from the rhizomes of Smilacina japonica were obtained through hot water extraction,Soxlets extraction and macroprorous adsorbing resin.Obtained water extraction, alcohol extraction, water solution of alcohol extraction from the rhizomes of Smilacina japonica, and Ⅰ,Ⅱ,Ⅲ,Ⅳ four parts of matter were isolated from alcohol extract by macroporous adsorbing resin method in turn with 30%,50%,70%,90% ethanol elution, and five flavonoids,3-methoxy-8-methylquercetin(A),8-methylluteolin(B),3′-methoxyluteolin(C),luteolin(D) and quercetin(E) were isolated from the Ⅲ.The method of phenanthroline-Fe~(2+)-H_2O_2 reaction system was used to measure scavenging effect to hydroxyl radical(HO·).The results showed that all extracts of Smilacina japonica had ability to scavenge hydroxyl radical,the scavenging ability was 23.30%-98.07% except alcohol extraction, the scavenging ability of five compounds were stronger even than that of Vit C in dose-dependant manner.Therefore, plant of Smilacina japonica has good antioxidant active ingredient.