龟背竹的离体培养和快速繁殖 |
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引用本文: | 韩德伟,王振龙,关丽霞.龟背竹的离体培养和快速繁殖[J].安徽农业科学,2010,38(26):14270-14271. |
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作者姓名: | 韩德伟 王振龙 关丽霞 |
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作者单位: | 辽宁农业职业技术学院,辽宁营口,115009;辽宁农业职业技术学院,辽宁营口,115009;辽宁农业职业技术学院,辽宁营口,115009 |
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摘 要: | 目的]寻找龟背竹组织培养的适宜培养基,建立成熟的组培快繁技术。方法]以龟背竹成年茎段扦插后生成的腋芽作为外植体进行离体培养,寻找合适的诱导培养基和增殖培养基,并进行生根培养。结果]确定龟背竹不定芽的最佳诱导培养基为MS+BA5.0mg/L(单位下同)+ZT0.5+NAA0.1,增殖的适宜培养基为MS+BA3.0+ZT0.3+NAA0.1,生根培养基为1/2MS+IBA0.5+NAA0.2。结论]为龟背竹工厂化育苗的有效进行提供了技术支持和参考。
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关 键 词: | 龟背竹 组织培养 培养基 |
Tissue Culture and Rapid Propagation of Monstera deliciosa |
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Institution: | HAN De-wei et al(Liaoning Agricultural Vocation-technical College,Yingkou,Liaoning 115009) |
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Abstract: | Objective] The aim was to find the optimum medium for the tissue culture of Monstera deliciosa and to establish mature tissue culture technology.Method]With axillary generated after Monstera deliciosa adult stem cuttings as explants to culture in vitro to find out the inducing medium,multiplication medium and carry out rooting culture.Result] The optimum medium for Monstera deliciosa induction was MS+BA 5.0 mg/L +ZT 0.5 +NAA 0.1.The appropriate medium for multiplication and the rooting were MS+BA 3.0+ZT 0.3+NAA 0.1 and 1/2MS+IBA 0.5+ NAA 0.2,respectively.Conclusion] The results could provide technique support and reference for Monstera deliciosa effective factory breeding. |
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Keywords: | Monstera deliciosa Tissue culture Medium |
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