| Serial Nuclear Transfer of Goat-Rabbit Interspecies Reconstructed Embryos |
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| 引用本文： | ZHANG Zhi-guo ZHANG Xiao-rong LIU Ya JING Ren-tao WANG Cun-li znmo Huan LI Bin CAO Chen-chong LI Dong-wei CHENG Li-zi. Serial Nuclear Transfer of Goat-Rabbit Interspecies Reconstructed Embryos[J]. 中国农业科学(英文版), 2005, 4(8): 629-633 |
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| 作者姓名： | ZHANG Zhi-guo ZHANG Xiao-rong LIU Ya JING Ren-tao WANG Cun-li znmo Huan LI Bin CAO Chen-chong LI Dong-wei CHENG Li-zi |
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| 作者单位： | [1]College of of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China [2]Medical Reproduction Centre of the First Affiliated Hospital, Anhui Medical University, Hefei 230022, P,R.China [3]College of Life Science, University of Science and Technology of China, Hefei 230022, P.R.China |
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| 摘 要： | The experiments of serial nuclear transfer were conducted between Boer goat and rabbit. The enucleated oocytes of rabbit were used as recipients while the blastomeres of goat morula was used as nuclear donor. The reconstructed embryos developing to morula were used as donor for serial cloning. As a result, two generations of reconstructed embryos were obtained, including 58 first generation reconstructed embryos and 14 second generation reconstructed embryos. The fusion rates were 79.5 and 70%, respectively, and there was no significant difference between them （P〉0.05）. The cleavage rates were 75.9 and 28.6% respectively with significant difference （P〈0.01）. No blastocyst was obtained from the second generation reconstructed embryos while 13.8% of first generation reconstructed embryos developed to blastocyst.
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| 关 键 词： | 山羊 兔子 迁移行为 晶胚 克隆技术 |
Serial Nuclear Transfer of Goat-Rabbit Interspecies Reconstructed Embryos |
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| ZHANG Zhi-guo,ZHANG Xiao-rong,LIU Ya,JING Ren-tao,WANG Cun-li,ZHAO Huan,LI Bin,CAO Chen-chong,LI Dong-wei,CHENG Li-zi. Serial Nuclear Transfer of Goat-Rabbit Interspecies Reconstructed Embryos[J]. 《Agricultural Sciences in China》, 2005, 4(8): 629-633 |
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| Authors: | ZHANG Zhi-guo ZHANG Xiao-rong LIU Ya JING Ren-tao WANG Cun-li ZHAO Huan LI Bin CAO Chen-chong LI Dong-wei CHENG Li-zi |
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| Abstract: | The experiments of serial nuclear transfer were conducted between Boer goat and rabbit. The enucleated oocytes of rabbit were used as recipients while the blastomeres of goat morula was used as nuclear donor. The reconstructed embryos developing to morula were used as donor for serial cloning. As a result, two generations of reconstructed embryos were obtained, including 58 first generation reconstructed embryos and 14 second generation reconstructed embryos. The fusion rates were 79.5 and 70%, respectively. and there was no significant difference between them (P＞0.05). The cleavage rates were 75.9 and 28.6% respectively with significant difference (P＜0.01). No blastocyst was obtained from the second generation reconstructed embryos while 13.8% of first generation reconstructed embryos developed to blastocyst. |
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| Keywords: | Goat Interspecies cloning Serial nuclear transfer |
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