| 口服急性毒性试验中Cry1C蛋白对鼠肠道菌群的影响 |
| |
| 引用本文： | 郭星,许文涛,姚业成,曹思硕,罗云波,黄昆仑.口服急性毒性试验中Cry1C蛋白对鼠肠道菌群的影响[J].农业生物技术学报,2010,18(2):346-351. |
| |
| 作者姓名： | 郭星 许文涛 姚业成 曹思硕 罗云波 黄昆仑 |
| |
| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083
2. 中国农业大学食品科学与营养工程学院,北京,100083;农业部转基因生物食用安全检验监督测试中心,北京,100083 |
| |
| 基金项目： | 国家自然科学基金，转基因专项 |
| |
| 摘 要： | 利用变性梯度凝胶电泳(DGGE)分析不同ICR(institute of cancer researcch)小鼠在口服急性毒性试验中摄入Cry1C蛋白前后其肠道菌群的变化,进而对该抗虫蛋白的食用安全性进行较为深入的研究.DGGE图谱经UPGAMA聚类分析显示:小鼠间菌群结构存在较明显的个体差异;实验期间,对照组小鼠灌胃前后其菌群基本保持稳定;实验组小鼠试验前后肠道菌群变化差异较对照组明显.随着灌胃的停止其变化差异在经历过高峰后会随着停喂时间的延长而减小,且菌相有逐渐恢复灌胃前结构的趋势.从肠道菌相的角度分析可知:该抗虫Cry1C蛋白对小鼠是基本安全的.
|
| 关 键 词： | Cry1C蛋白 口服急性毒性 肠道菌群 变性梯度凝胶电泳 转基因水稻 |
Analysis of Intestinal Microbiota in Mice Fed with Cry1C Protein in Acute Oral Toxicity Test |
| |
| Guo Xing,Xu Wentao,Yao Yecheng,Cao Sishuo,Luo Yunbo,Huang Kunlun.Analysis of Intestinal Microbiota in Mice Fed with Cry1C Protein in Acute Oral Toxicity Test[J].Journal of Agricultural Biotechnology,2010,18(2):346-351. |
| |
| Authors: | Guo Xing Xu Wentao Yao Yecheng Cao Sishuo Luo Yunbo Huang Kunlun |
| |
| Institution: | Guo Xing 1 Xu Wentao 1,2 Yao Yecheng 1 Cao Sishuo 1 Luo Yunbo 1 Huang Kunlun 1,2 1 College of Food Science , Nutritional Engineering,China Agricultural University,Beijing,100083,2 Supervision,Inspection , Testing Center of Genetically Modified Orginisms,Ministry of Agriculture |
| |
| Abstract: | In this paper, intestinal microbiota of ICR(institute of cancer researcch) mice fed with Cry1C protein in acute oral toxicity test were analyzed by denaturing gradient gel electrophoresis(DGGE), furthermore, edible safety of insect-resistant protein was evaluated in deepth.DGGE maps by UPGAMA cluster analysis indicated that the intestinal flora among the mice showed significant difference;the mouse in the control group had unique and stable intestinal flora in the test period;the intestinal flora of the mou... |
| |
| Keywords: | Cry1C protein Acute oral toxicity Intestinal microbiota Denaturing gradient gel electrophoresis Genetically modified rice |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
