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| 农杆菌介导北方粳稻转化体系的研究 | |
| 引用本文： | 刘双奇,邹德堂,孟英,卢泳全,卞景阳. 农杆菌介导北方粳稻转化体系的研究[J]. 黑龙江农业科学, 2008, 0(4): 6-8 |
| 作者姓名： | 刘双奇 邹德堂 孟英 卢泳全 卞景阳 |
| 作者单位： | 1. 东北农业大学农学院,哈尔滨,150030 2. 黑龙江省农业科学院耕作栽培研究所,哈尔滨,150086 3. 黑龙江省农业科学院生物技术研究所,哈尔滨,150086 |
| 摘 要： | 通过农杆菌介导的方法将Bar-Bt基因（抗除草剂基因和抗虫基因构建在同一载体上的双基因）整合到北方优质粳稻龙稻6号、松粳9号等品种中;分别以供试品种的幼胚和成熟胚为外植体诱导愈伤组织,携带有Bar-Bt基因双元载体PCAMBIA1301号的根癌农杆菌EHA105为载体,GUS基因的瞬时表达率为衡量指标,对影响农杆菌介导的龙稻6号、松粳9号等品种的转化效率的主要因素进行了研究。结果表明：农杆菌侵染愈伤组织的菌液浓度以D（600nm）=0.8～1.0较为适宜,最佳共培养时间为2～3 d,预培养时间为5 d,共培养培养基中添加100μmol·L^-1乙酰丁香酮的转化效率有明显提高。 |
| 关 键 词： | 粳稻 农杆菌 遗传转化 GUS瞬时表达率 |
Establishment of Transformation System Mediated by Agrobacterium Tumefaciens in Grain-oryza of North of China |
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| LIU Shuang-qi,ZOU D-etang,MENG Ying,LU Yong-quan,BIAN Jing-yang. Establishment of Transformation System Mediated by Agrobacterium Tumefaciens in Grain-oryza of North of China[J]. Heilongjiang Agricultural Science, 2008, 0(4): 6-8 | |
| Authors: | LIU Shuang-qi ZOU D-etang MENG Ying LU Yong-quan BIAN Jing-yang |
| Affiliation: | LIU Shuang-qi , ZOU De-tang , MENG Ying, LU Yong-quan , BIAN Jing-yang (1. Agronomy College of Northeast Agricultural University, Harbin 150030;2. Crop Tillage and Cultivation Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086;3. Biotechnological Research Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086) |
| Abstract: | With juvenile embryo of grain-oryza of North of China as explant, Agrobacterium tumefaciens strain EHAl05 carrying Tiplasmid PCAMBIA1301 with Bar-Bt gene as vector,and the transient expression rate of GUS gene as measuring index,main factors that affected transformation efficiency of grain-oryza of North of China mediated by Agrobacterium tumefaciens were studied in this article. Results showed that the proper concentration of bacteria solution to infect callus was D（600nm）-0.8～1.0,the most suitable co-cultivation time was 2～3 days, pre-cultivation time was 5 days, and addition of 100μmol·L^-1 Acetosyringone improved transformation efficiency considerably. |
| Keywords: | Japonica rice Agrobacterium tumefaciens genetic transformation transient expression of GUS gene |
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