Cellulolytic activity and virulence of Ophiostoma ulmi and O. novo‐ulmi isolates |
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| Authors: | K Przyby? H Dahm A Ciesielska K Moliński |
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| Institution: | 1. Polish Academy of Sciences, Institute of Dendrology, 62‐035 Kórnik, Poland. E‐mail: kmtprz@man.poznan.pl;2. Department of Microbiology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Toruń;3. Department of Mathematical and Statistical Methods, University of Agriculture, Poznań, Poland |
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| Abstract: | The cellulolytic activity (exoglucanase, endoglucanase and β‐glucosidase) of Ophiostoma ulmi (four isolates), O. novo‐ulmi (19 isolates) and ‘fast‐waxy’ (five isolates) was determined in growth media containing carboxymethylcellulose (CMC) and cellulose powder. Differences in enzyme activities were observed among isolates, irrespective of the species and substrate used. Inoculation experiments on Ulmus minor with randomly selected isolates of O. ulmi (two isolates), O. novo‐ulmi (five isolates) and ‘fast‐waxy’ (two isolates) were also performed. Disease was assessed as the percentage of leaves showing yellowing and browning. Ophiostoma novo‐ulmi and ‘fast‐waxy’ isolates exhibited a great variability in their capacities to cause the disease. In the presence of CMC, a significant correlation between the activity of exoglucanase and β‐glucosidase in vitro and virulence was found. |
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