| 大豆胰蛋白酶抑制剂KSTI3基因的克隆及其植物表达载体的构建 |
| |
| 引用本文： | 吕品,柴晓杰,王丕武,张宇.大豆胰蛋白酶抑制剂KSTI3基因的克隆及其植物表达载体的构建[J].吉林农业大学学报,2007,29(3):275-278. |
| |
| 作者姓名： | 吕品 柴晓杰 王丕武 张宇 |
| |
| 作者单位： | 吉林农业大学生物技术学院,长春,130118;大连水产学院生命科学与技术学院,大连,116023 |
| |
| 基金项目： | 吉林省重大科技攻关项目(20040203-2-2) |
| |
| 摘 要： | 以大豆基因组DNA为模板,利用聚合酶链式反应(PCR)技术克隆了大豆胰蛋白酶抑制剂基因KSTI3的全长DNA片段,并将其构建到pMD18-T vector上。核苷酸序列测定结果表明:该基因片段全长654 bp,与已发表的KSTI3基因序列同源性达99%。将反义 正义基因片段插入到pBI121 35S启动子下,构建重组质粒pBIKSTI3。通过冻融法将该重组质粒转入根癌农杆菌EHA105中,获得了植物表达载体。
|
| 关 键 词： | 胰蛋白酶抑制剂 PCR 克隆 植物表达载体 大豆 |
| 文章编号： | 1000-5684（2007）03-0275-04 |
| 修稿时间： | 2006-06-252006-09-15 |
Cloning and Construction of Expression Vector on KSTI3 Gene of Soybean Kunitz-type Trypsin Inhibitor |
| |
| L Pin,CHAI Xiao-Jie,WANG Pi-wu,ZHANG Yu.Cloning and Construction of Expression Vector on KSTI3 Gene of Soybean Kunitz-type Trypsin Inhibitor[J].Journal of Jilin Agricultural University,2007,29(3):275-278. |
| |
| Authors: | L Pin CHAI Xiao-Jie WANG Pi-wu ZHANG Yu |
| |
| Institution: | 1. College of Biotechnology, Jilin Agricultural University, Changchun 130118, China ; 2. School of Life Science and Technology, Dalian Fisheries University, Dalian 116023, China |
| |
| Abstract: | Soybean Kunitz-type trypsin inhibitors are important antinutritious factors.The full-length DNA fragment of soybean Kunitz-type Trypsin Inhibitor gene KSTI3 was amplified by PCR technique,then PCR products were cloned into pMD18-T vector.Nucleotide sequence analysis showed that the cloned fragment consisted of 654 bp and shared 99%identity with the reported KSTI3.The fragment of anti-sense gene plus-sense gene was constructed to the downstream of 35 S promoter in the binary vector pBI121,which formed the recombinant plasmid pBIKSTI3. The plasmid pBIKSTI3 was mobilized into Agrobacterium tumefaciens strain EHA105 by freeze-melting methods,and a plant expression vector was thereby obtained. |
| |
| Keywords: | Kunitz-type trypsin inhibitor PCR cloning plant expression vector soybean |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
