| 桃AFLP技术反应体系的优化 |
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| 引用本文： | 赵成日,曹后男,朴钟云,宗成文,王成. 桃AFLP技术反应体系的优化[J]. 吉林农业大学学报, 2007, 29(4): 394-397 |
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| 作者姓名： | 赵成日 曹后男 朴钟云 宗成文 王成 |
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| 作者单位： | 延边大学农学院,龙井,133400;日本岐阜大学应用生物科学部,岐阜,5011193;延边大学农学院,龙井,133400;沈阳农业大学园艺学院,沈阳,110161;延边大学农学院,龙井,133400;南京农业大学园艺学院,南京,210095 |
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| 基金项目： | 国家自然科学基金(30160054) |
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| 摘 要： | 以"珲春桃"与"正满早生"杂交获得的32株F_1代群体为材料,对影响桃AFLP技术分析体系的因素作了详细的探讨,优化出一套稳定、可靠的桃AFLP反应体系。经优化,得出最适宜的结果:酶切时间为6h、Mg- Cl_2浓度为1.75 mmol/L、预扩增产物稀释倍数为10倍。利用优化后的桃AFLP分析体系对该技术的重复性进行了验证,重复率可达90%。
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| 关 键 词： | 桃 AFLP 分析体系 |
| 文章编号： | 1000-5684（2007）04-0394-04 |
| 修稿时间： | 2006-09-072006-11-23 |
Screening of AFLP Reaction System in Peach |
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| ZHAO Cheng-ri,CAO Hou-nan,PIAO Zhong-yun,ZONG Cheng-wen,WANG Cheng. Screening of AFLP Reaction System in Peach[J]. Journal of Jilin Agricultural University, 2007, 29(4): 394-397 |
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| Authors: | ZHAO Cheng-ri CAO Hou-nan PIAO Zhong-yun ZONG Cheng-wen WANG Cheng |
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| Affiliation: | 1. College of Agriculture, Yanbian University, Longjing 133440, China ; 2. Faculty of Applied Biolog- ical Sciences, Gifu University, Gifu 5011193, Japan ; 3. College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; 4. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | In this study,32 plants of F_1 progeny,obtained by crossing Hunchun peach and Zhengmanza- osheng,were used as materials to study the factors influencing AFLP analysis system in peach.A set of steady and credible system was optimized.Through optimization,the best results are proven to be:diges- tion time 6 hours,concentration of MgCl_2 1.75 mmol/L and dilution times of pre-amplification product 10 times.The experiment of testing the repetition of the technique was conducted using the optimized AFLP analysis system,and the frequency reached 90% |
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| Keywords: | peach AFLP analysis system |
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